摘要
目的建立一种便捷灵敏的青蒿素含量测定方法，并测定其在不同溶剂中的溶解度及油水分配系数、方法均匀试验设计优化青蒿素的柱前衍生条件，采用HPLC—ECD法进行测定。结果优化的青蒿素柱前衍生条件为：1mL青蒿素无水乙醇溶液与4mL氢氧化钠0．01mol·L-1于70℃水浴中反应50min后，冷却至室温，再加入5mL醋酸0．06mol·L-1。青蒿素在0．0015-0．7500μg范围内线性关系良好。结论建立的柱前衍生HPLC—ECD法专属性强、灵敏度高，适用于青蒿素的测定。
Objective To establish a sensitive assay oil-water partition coefficient in different solvents. optimized by uniform design, and the determination of precolumn derivatization conditions were as follows： 0.01 mol·L-1 sodium hydroxide were reacted in 70 ℃ artemisinin, and to detect the solubility as well as its Methods The pre-colnmn derivatization of artemisinin was artemisinin was performed by HPLC-ECI）. Results The optimal one milliliter of artemisinin anhydrous ethanol solution and 4 mL water bath for 50 min, and then cooled to room temperature with 5 mL of 0.06 mol ·L-1 acetic acid added. The content of artemisinin showed good linearity in the range of 0.0015-0.7500 μg. Conclusion pre-eohmm derivatization HPLC-ECD method is highly specific and sensitive, and can be used for the assay of artemisinin
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2014年第3期352-356,共5页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
中柬第三方检测平台建设项目(2012年广州市白云区国际科技合作计划项目)(2012-KQ-23)
