灯盏花素对脑缺血再灌注大鼠海马核因子-κB表达的影响

The effect of breviscapine on the expression of NF-κB in hippocampus of cerebral ischemia-reperfusion rat

目的探讨灯盏花素(Br)对脑缺血再灌注大鼠海马核因子(NF)-κB表达的影响。方法采用Zea-Longa法建立脑缺血再灌注动物模型。96只SD大鼠根据不同的处理因素分为:假手术组(Sham组,32只)、灯盏花素治疗组(Br组,32只)、缺血再灌注组(I/R组,32只)。Br组和I/R组依据缺血后再灌注的不同时间点再细分为3、12、24、48h4个小组。应用免疫组织化学方法和蛋白印迹方法检测各组大鼠海马NF-κB蛋白的表达。结果与假手术组相比,在缺血再灌注3h时,大鼠海马NF-κB的阳性表达已明显升高,在12h时表达最高,随后表达开始下降(P<0.05);与相同时间点的缺血再灌注组大鼠比较,3h时,Br组的NF-κB蛋白阳性表达未见明显降低(P>0.05),其余各时间点则明显降低(P<0.05);蛋白印迹方法也得到了相同的结论。结论灯盏花素很可能通过减少NF-κB蛋白的表达改善脑缺血再灌注损伤。

Objective To study the expression of NF-κB in hippocampus of cerebral ischemia-reperfusion rat after breviscapine was injected.Methods The ischemia-reperfusion model was induced with Zea-Longa′s way.Ninty-six SD rats according to the different treatment were randomly divided into the sham group,ischemia-reperfusion group（I/R）and breviscapine group（Br）at different time points（3h,12h,24h,48h）after reperfusion on average.The expression of NF-κB in hippocampus of all groups was examined by immunochemistry and western blot methods.Results Compared with the sham group,the positive expression of NF-κB in hippocampus of the rats in I/R group was increased significantly at the point of 3hafter reperfusion（P〈0.05）,to the peak at 12h;compared with the rats in I/R group at the same time point,the positive expression of NF-κB didn′t evidently reduce at the point of 3hafter breviscapine was injected（P〉0.05）,and decreased significantly in 12h,24hand48hgroups（P〈0.05）;and the same result was obtained by western blot method.Conclusion Breviscapine could down-regulate the expression of NF-κB in hippocampus of cerebral ischemia-reperfusion rat.