多孔钽金属与成骨细胞生物相容性的实验研究

In vitro biocompatibility study of the osteoblast response to porous tantalum

[目的]研究多孔钽金属体外与成骨细胞的生物相容性。[方法]按照ISO10993-12医疗器械生物学评价标准制备多孔钽金属材料浸提液,作用于人成骨细胞系hFOB1.19,标记为实验组,并设置空白对照组;MTT法检测材料浸提液对成骨细胞生长的影响;ELISA酶联免疫吸附法检测实验组及对照组碱性磷酸酶ALP及骨钙素OCN活性;将成骨细胞与多孔钽金属共培养,扫描电镜观察成骨细胞在材料上黏附和生长情况。[结果]实验组和对照组细胞增殖曲线、ALP和OCN活性相似,差异无统计学意义(P>0.05);成骨细胞与多孔钽金属材料共培养,显现出良好的黏附和生长行为。[结论]多孔钽金属对成骨细胞生长无不良影响,且适于成骨细胞黏附、生长及分化,具有良好的生物相容性。

[ Objective] To investigate the in vitro biocompatibility of porous tantalum on osteoblasts. [ Method] A leaching solution of porous tantalum was prepared according to the standards set forth in the biological evaluation of medical devices （ISO10993 -12）. The osteoblasts were divided into 2 groups：a blank control group and an experimental group, in which the porous tantalum solution acted on human osteoblasts （hFOB1. 19 ）. The MTT method was used to measure the effects of the leaching solution on the cells. Enzyme - linked immunosorbent assay method was used to detect the activity of ALP and OCN in the experimental and blank control groups. The osteoblasts were cultured with porous tantalum, and a scanning electron microscope was used to observe cell adhesion and growth on the porous tantalum. [ Result] The study showed that the cell proliferation curve, ALP, and OCN of the experimental and blank control groups were similar （ P 〉 0.05 ）, and the osteoblasts had good adhesion and growth behavior on the porous tantalum. [ Conclusion ] Porous tantalum did not have any adverse effects on osteoblast activity. It demonstrated good biocompatibility and is a good metal for the attachment, growth, and differentiation of osteoblasts.