动脉移植BMSCs对大鼠缺血再灌注损伤脊髓CNTF和STAT3的影响

Effects of the bone marrow mesenchymal stem cells transplantated by Abdominal aortic on CNTF and STAT3 in spinal cord ischemic reperfusion injured rats

目的探讨肾下腹主动脉移植骨髓间充质干细胞（BMSCs）对大鼠缺血再灌注损伤脊髓CNTF和STAT3的影响及对损伤脊髓功能恢复的作用。方法24只成年SD雌性大鼠随机分为假手术组、对照组及移植组3组，每组8只。术后对大鼠进行后肢神经行为学评分和运动诱发电位检测，采用RT—PCR、Westernblot检测大鼠缺血节段脊髓内CNTF和STAT3的表达变化。结果与假手术组比较，对照组和移植组BBB评分于术后0d、5d、10d、15d显著降低（P〈0．01），MEP潜伏期延长（P〈0．01）、波幅减小（P〈0．01），脊髓CNTF和STAT3mRNA和蛋白表达增加（P〈0．01）；与对照组比较，移植组BBB评分于术后5～15d增高（P〈0．01），MEP潜伏期缩短（P〈0．01）、波幅增加（P〈0．01），CNTF和STAT3mRNA和蛋白表达增加（P〈0．01）。结论肾下腹主动脉移植BMSC可能通过增加损伤脊髓局部CNTF和STAT-3表达促进脊髓缺血再灌注损伤大鼠后肢功能恢复。

Objective To investigate the functional recovery and effects of the bone marrow mesenchymal stem cells （BMSCs） transplanted by abdominal aortic on CNTF and STAT3 in spinal cord ischemic reperfusion injured （SCIRI） rats. Methods 24 adult female SD rats were assigned randomly to 3 groups of sham operation group, control group and transplantation group （8 rats in each group）. The neurological functional status of animal was assessed with BBB scores and mo- tor evoked potentials （MEP） after the operation. After last scoring, RT-PCR and Western blot were used to detect the expressional changes of CNTF and STAT3 in the lumbar segments of spinal cord. Results Compared with the sham group, in the SCIRI rats,the BBB scores were markedly decreased at 0 d,5 d,10 d and 15 d （P 〈0.01 ） ,the latency and the amplitude of MEP was longer and lower at 15d post operation respectively （P 〈 0.01 ） , the mRNA and protein level of CNTF and STAT3 were increased obviously （P 〈 0.01 ）. Compared with the control group, the BBB scores were increased at 5 d, 10 d and 15 d （P 〈 0.01 ） ,the latency was shorter and the amplitude was higher （ P 〈 0.01 ） , the mRNA and protein level of CNTF and STAT3 were increased in the transplanted rats（P 〈 0.01 ）. Conclusion BMSCs,transplanted by the abdominal aorta, can promote the expression of CNTF and STAT3 in the injuried spinal cord and improve the hind limb function recovery in SCIRI rats.