羊输卵管上皮细胞对小鼠受精卵体外发育的影响

Effect of Goat Oviductal Epithelial Cells on the in-vitro Development of Mouse Embryos

为探讨输卵管上皮细胞对小鼠雌雄原核受精卵 ( 2 PN受精卵 )体外发育的影响 ,首先建立了羊输卵管上皮细胞共培养体系 ,再将小鼠 2 PN受精卵分别放在 M1 6培养液 (对照组 )中和羊输卵管上皮细胞 +M1 6培养液 (实验组 )中培养。结果表明 :在对照组中 ,只有 50 %的 2 -细胞胚胎能克服体外发育的阻断而达到 4-细胞阶段 ,发育到囊胚期的比率仅为 2 8.2 %。实验组中 ,有 86.75%的 2 -细胞胚胎可以克服体外发育的阻断而达到 4-细胞阶段 ,发育到囊胚期的比率为 45.7%。对照组与实验组相比 ,统计学上有显著差异 ( P<0 .0 1 )。这提示 :输卵管上皮细胞有助于小鼠胚胎克服体外发育阻断 。

To investigate the effect of oviductal cells system on the in vitro development of mouse 2PN fertilized eggs , the co culture system of goat oviductal epithelial cells were established. Mouse 2PN fertilited eggs were co cultured with goat oviductal epithelial cells in M16 co medium(test group) or in M16 medium alone(control group) . The results showed that more than 86% of embryos co cultured with the oviductal cells cleaved beyond the 'in vitro' block whereas only 50% of embryos in M16 medium cleaved to the same degree ( P <0.01) . The findings suggested that the goat oviductal epithelial cells system had the beneficial effect on mouse 2PN fertilized eggs passing the '2 cell block' in vitro .