应用正交设计方法优化香蕉外植体直接出芽的条件

OPTIMIZATION FOR DIRECT BUDDING FROM EXPLANTS IN BANANA USING ORTHOGONAL DESIGN

利用正交设计方法［Ｌ２７（３１３）］研究了培养基中的植物生长调节物ＢＡＰ、ＮＡＡ和ＩＢＡ，以及不同外植体对香蕉（Ｍｕｓａ ａｃｕｍｉｎａｔａ Ｃｏｌｌａ ｃｖ． Ｗｉｌｌｉａｍｓ）直接出芽频率的影响。外植体的不同是影响香蕉直接出芽频率的最主要因素，ＮＡＡ和ＩＢＡ对实验结果的影响相同，均弱于ＢＡＰ。实验结果的统计分析表明，诱导香蕉外植体直接出芽的优选培养基为不添加ＢＡＰ、ＮＡＡ和ＩＢＡ的ＭＳ基本培养基，最佳外植体为芽的顶端分生组织部分。

Orthogonal experimental design was adopted to tissue culture in banana (Musa acuminata Colla cv. Williams). Effects of BAP, NAA, IBA and different explants on explant direct budding were investigated in banana by using orthogonal design [L27 (313)]. Statistic analyses indicated that the main factor which mostly influenced the results was explants. BAP was the second factor, and both NAA and IBA the third. The results showed that the optimal medium was MS basal medium supplemented without the three growth regulators, and the best explant for the medium was the apical meristem of shoots.