微卫星BAT-26位点单态性的分析

Monomorphic Analysis of Microsatellite Loci at BAT-26

目的 微卫星不稳定性 (Microsatellite Instability,MSI)在许多肿瘤的发生中具很重要的作用 ,大多数微卫星 (Microsatellite,MS)位点在正常人群中多态性明显 ,有报道表明 BAT- 2 6位点单态性很好 ,据此我们分析了 60例正常肠粘膜标本中 BAT- 2 6位点 MSDNA的情况 ,以便了解 BAT- 2 6位点单态性情况。方法 组织 DNA提取后 PCR扩增 ,扩增产物行 6%变性聚丙烯酰胺凝胶电泳 ,最后银染。结果 所有标本在 BAT- 2 6位点 MSDNA长度无明显改变。结论 BAT- 2 6具有较好的单态性。

Objective MSI plays an important role in carainogenesis of mant tumors.Most MS loci show polymorphism in normal tissues.It has been reported that BAT\|26 was quasi\|monomorphic,showing only minor size variathions.To analysis monomorphism at BAT\|26 loci,60 normal colorectal tissues were examined.Methods Genomic DNA extracted from tissues was PCR amplified.PCR products were run on 6% denaturing polyacrylamide gel and stained with silver.Results All specimens showed no variation in the length of MS DNA at BAT\|26 loci.Conclusion BAT\|26 has much monomorphism in human genome.