摘要
目的 :用高效液相色谱 -蒸发光散射检测法 (HPLC -ELSD)直接测定银杏叶中银杏萜类内酯A、B、C及白果内酯的含量。方法 :银杏叶 10 %甲醇提取液通过聚酰胺及硅胶小柱联用净化制成供试品溶液 ,经C1 8色谱柱 ,以甲醇 -水为流动相 ,梯度洗脱 ,以蒸发光检测器检测。结果 :银杏叶供试品溶液的预处理是获得准确测定结果的关键 ,多点校正 ,自然对数换算以弥补ELSD线性应答差的不足。结论 :测定不同产地、不同树龄、不同采收季节的银杏叶中萜类内酯含量 ,对从源头抓好原料质量有积极意义。
Objective:Ginkgolide A,B,C and bilobalide in Ginkgo biloba leaves are determined simultaneously by RP-HPLC-ELSD.Method:10% methanolic extracts of the leaves are cleaned-up by solid phase extraction via polyamide cartridge and silica gel cartridge successively and the sample solutions are prepared.RP-HPLC analysis is carried out through C 18 column,methanol-water as mobile phase,eluted in gradient mode,detected by evaporated light scattering detector.Result:The key step for getting precise data is pre-treatment of the leaves extract through normal phase SPE cartridge;The poor linear response of ELSD can be compensated by multilevel calibration and logarithm calculation.Conclusion:The Ginkgo biloba leaves obtained from different area,different growth years and collection seasons are determined.It is significant for quality control of the herbal medicine initiated from the raw material.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2001年第3期173-176,共4页
Chinese Journal of Pharmaceutical Analysis
