对酞内酰胺苯甲酰氯荧光衍生法液相色谱测定苯酚和氯代酚

High Performance Liquid Chromatographic Fluorescence Determination for Trace Phenol and Chlorinated Phenols by Precolumn Derivatization with 4-(2-Phthalimidyl)Benzoyl Chloride

近年来气相色谱法、薄层色谱法和高效液相色谱法已成为应用最广的测定酚类的方法。Tsuruta等提出了对酞内酰胺苯甲酰氯(4-2(phthalimidyl)benzoyl chloride,简称PIB-Cl)的合成方法,并将PIB-Cl作为胺类和醇类化合物的荧光衍生化试剂,但将它与酚类化合物进行衍生反应及相应的色谱应用还未见报道。本文提出了PIB-Cl与酚的反应。

The present paper covers a simple, rapid and sensitive method for the determination of trace phenol and chlorinated phenols by HPLC. The phenols were derivatized by the reaction with 4-(2-ph-thalimidyl)benzoyl chloride very rapidly to produce the corresponding fluorescent esters, which can be separated on ODS column with aqueous acetonitrile as eluent. The eluates were quantitated by fluorescence detection with peak height method. The detection limits for the phenols were 0. 08~0. 16 pmol.