耳廓假性囊肿与机体免疫功能的关系

Research on the immunological cause of auricular pseudocyst

目的 :探讨耳廓假性囊肿发病与机体免疫功能的关系。方法 :采集 5 5例耳廓假性囊肿患者的囊液和血清 ,分别用免疫单扩散法、聚乙二醇比浊法、间接荧光抗体技术和免疫转印技术等检测其中的抗体 Ig G、Ig A、Ig M,补体 C3 ,免疫复合物以及抗核抗体、ENA多态抗体等的含量 ;用免疫组织化学方法检测患者囊壁组织中的免疫复合物。以患者血清、囊液以及正常人血清分别与实验大鼠耳廓和正常胎儿耳廓组织作用后观察反应情况。结果 :耳廓假性囊肿患者囊液中抗体和补体含量均明显低于血清中含量 (P <0 .0 1) ,在患者病变耳廓软骨组织上发现了免疫复合物沉积。患者囊液与正常胎儿耳廓组织作用后也发现了免疫复合物沉积。结论 :耳廓假性囊肿的发生可能与机体局部自身免疫有一定关系。

Objective:To elucidate the relationship between the auricular pseudocyst and the immunological function of patients.Method:The cyst fluid and blood sample had been detected for contents of IgG, IgA, IgM and complement C 3 by radial immunodiffusion, immunocomplex (IC) contents by polyethylene glycol turbidimetry in 55 cases, anti nuclear antibody (ANA) by immuno fluorescent technique in 23 cases, extractable nuclear antibody (ENA) by immunotransfer technique in 24 cases.The frozen sections of cyst wall tissue of 24 cases had been detected for immunocomplex by immunoenzyme histochemistric method.The reactions were observed separately between the auricle tissue of healthy white rat, humman embryo and cyst fluid, serum of patient and normal serum.Result:Contents of IgG, IgA, IgM and C 3 in the cyst fluid were lower than the serum significantly (P< 0.01 ).No IC, ANA and ENA could been found both in serum and cyst fluid, but reactive immunocomplex was discovered on the aurical cartilagous tissue of patient, immunocomplex also be observed on human embryo aurical tissue when it reacted with cyst fluid,but complex hadn′t be found when the auricle tissue of white rat and human embryo reacted with serum of patient and normal people.Conclusion:The reason of auricular pseudocyst may be related with the local autoimmune status of patient.