对氨基苯甲酸对变形链球菌在唾液获得性膜上粘附的影响

Effect of Para aminobenzoic Acid on the Adhesion of Streptococcus Mutans

目的 探讨对氨基苯甲酸 (Para- aminobenzoic acid,PABA)对变形链球菌 (简称变链菌 )粘附至唾液包被羟磷灰石 (S- HA)的影响。方法 采用 Gibbons创立的体外粘附实验模型 ,以 3 H-胸腺嘧啶核苷 (3 H- Td R)为标记对变链菌的粘附进行定量观察。结果 不同浓度 PABA溶液 (10 - 9～ 10 - 3 g/ L)处理的变链菌在 S- HA粘附测得的放射性测量计数率 (cpm)与阳性对照组差异有显著性 ,即 10 - 9～ 10 - 3 g/ L 的 PABA溶液对变链菌在 S- HA粘附产生显著的抑制作用 ,但这种抑制作用在 PABA浓度增加到 10 - 3 g/ L 时则下降。结论 当 PABA的浓度为10 - 9～ 10 - 3 g/ L时能对变链菌在 S- HA粘附产生显著抑制作用。

Objective To investigate the effects of Para aminobenzoic acid (PABA) on the adhesion of Streptococcus mutans to saliva coated hydroxyapatite (S HA). Methods The adhesion model in vitro established by Gibbons was adopted in this study to quantify adsorptive cells through thymidine labelling. Results PABA solution over a range of concentration from 10 -9 to 10 -3 g/L could inhibit the attachment of S.mutans to S HA effectively, and the inhibition declined when the concentration of PABA solution was 10 -3 g/L. Conclusion PABA can inhibit the adherence of Streptococcus mutans to S HA effectively.