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人肺癌CD44蛋白和基因的异常表达与淋巴结转移的相关性 被引量:13

Correlation between Abnormal Expressions of CD44 Protein and Gene and Lymph Node Metastasis for Human Lung Carcinoma
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摘要 目的:研究CD44蛋白和基因在人肺癌组织中的异常表达,以及这种异常表达与淋巴结转移的相关性。方法:应用SP免疫组织化学染色方法和RT-PCR/Southern方法,研究人肺癌组织中CD44蛋白和基因的表达,并应用 QTM970计算机图像分析仪,对免疫组织化学染色结果进行定量分析。结果:SP免疫组织化学染色和计算机图像定量分析结果显示,非小细胞肺癌(non-small cell lung carcinomas, NSCLCs)组织比癌旁肺组织,有淋巴结转移比无淋巴结转移的非小细胞肺癌组织染色强度深,差异有极显著意义(F=16.67,P<0.01);RT-PCR/Southern杂交结果显示,有淋巴结转移较无淋巴结转移的非小细胞肺癌表达更多的 CD44 mRNA异常转录子(X2=12.13, P<0.01)。结论:CD44蛋白和基因在人非小细胞肺癌中出现异常表达,这种异常表达在人非小细胞肺癌的发展和淋巴结转移中起重要作用。检测CD44蛋白和基因的表达,可能对判断非小细胞肺癌病人的预后、预测淋巴结的转移趋势,具有重要的临床意义。 Objectives: The aim of this study was to investigate the abnormal expressions of CD44 protein and gene, relationship between the abnormal expressions of CD44 protein and gene and lymph node metastasis of human lung carcinomas. Methods: SP immunohistochemistry and RT-PCR/Southern methods were used to investigate the expressions of CD44 protein and gene of human lung carcinomas. The immunohistochemistry results were quantitatively analyzed with QTM970 computed image analyzer. Results: SP immunohistochemistry results and quantitative analysis revealed that stronger expression of CD44 protein was observed in non-small cell lung carcinomas than in para-carcinomatous pulmonary tissues, in non-small cell lung carcinomas with lymph node metastasis than in non-small cell lung carcinomas without lymph node metastasis (F = 16. 67, P < 0. 01 ). RT-PCR/Southern results shew that more abnormal CD44 mRNA transcripts were observed in the non-small cell lung carcinomas (NSCLCs) with lymph nodes metastasis than in non-small cell lung carcinomas without lymph node metastasis(X2 = 12. 13, P < 0. 01 ). Conclusions: The abnormal expressions of CD44 protein and gene were observed in non-small cell lung carcinomas. The abnormal expressions are not only involved in the development, but also in the lymph nodes metastasis of non-small cell lung carcinomas. CD44 expressions may have significant clinical importance in the evaluation of the patient prognosis and the potential tendency of lymph nodes metastasis.
出处 《癌症》 SCIE CAS CSCD 北大核心 2001年第6期595-598,共4页 Chinese Journal of Cancer
关键词 肺肿瘤 CD44 淋巴结转移 基因异常 Lung carcinoma, CD44 Lymph node metastasis
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参考文献2

  • 1Kuo M Y,J Oral Pathol Med,1998年,27卷,428页
  • 2Harn H J,Cancer,1995年,75卷,1065页

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