摘要
以ATCC14994为出发菌株 ,采用紫外线与亚硝基胍相结合的多次诱变育种 ,获得 1株 5′ 磷酸二酯酶高产菌株HAT2 2 2 8。通过单因子和正交试验对该菌株的产酶发酵条件进行了优化 ,优化发酵产酶条件为 :蔗糖 5 %,酵母膏 0 .3%,蛋白胨 0 .3%,K2 HPO4 0 .8%,KH2 PO4 0 .8%,MgSO4 0 .2 %,ZnSO4 0 .2 %,培养基起始 pH6 .0 ,接种量10 %,培养温度 30℃ ,摇床转速 12 0r/min ,发酵时间 48h。在优化条件下 ,HAT2 2 2 8的产酶水平达 132
A high 5′-phosphodiesterase producing mutant was screened from ATCC14994 after treated with UV and NTG. Medium for fermentation and conditions were optimized using single factor and orthogonal experiments. Under optimum medium the enzyme production level was at 1329μ/ml.
出处
《微生物学杂志》
CAS
CSCD
2001年第3期28-30,共3页
Journal of Microbiology