薄层色谱法测定消毒液和合成产品中的3,5-二甲基对氯苯酚

Determination of 4-Chloro-3,5-Dimethyl Phenol in Antiseptics and Synthetic Products by Scanning Thin-Layer Chromatography

对氯代二甲苯酚类化合物的薄层色谱分离条件进行了研究 ,建立了以苯 氯仿 (体积比为 2∶3 )的混合溶剂为展开剂 ,以GF2 54薄层板为层析板 ,直接测定 3 ,5 二甲基对氯苯酚含量的薄层色谱扫描方法。其被测组分的质量浓度在 0 185 8g/L～ 7 4 3 2 0g/L范围内与相应的峰面积的线性关系良好 ,相关系数为 0 9994。利用该方法对市售消毒液及氯代二甲苯酚类化合物合成过程中的题示化合物进行分析 。

A new method of scanning thin layer chromatography was established for the quantitative determination of the 4 chloro 3,5 dimethyl phenol by developing with 40% benzene in trichloromethane on GF 254 silica gel plate The results showed that the linear dynamic range of 4 chloro 3,5 dimethyl phenol was 0 185?8 g/L 7 432?0 g/L in which the relative peak area obeyed the Lambert Beer's law with a correlation coefficient of 0 999?4 and recoveries of 98 31% 100 1%. The method is simple, rapid, sensitive and reproducible