2型糖尿病患者β_3-肾上腺素能受体基因突变与肥胖的关系

Association of β_3-adrenergic receptor gene with obesity in patients with type 2 diabetes mellitus

目的 研究 2型糖尿病患者 β3 肾上腺素能受体基因突变与肥胖的关系。方法 采用聚合酶链反应 限制性内切酶片段长度多态性 (PCR RFLP)技术检测了 15 4例 2型糖尿病患者 β3 肾上腺素能受体基因Trp6 4Arg错义突变 ;同时检查体质指数、腰臀比值、血压及测定血脂等指标。结果 糖尿病伴有肥胖者Trp6 4Arg基因型的频率为 42 5 % ,Arg6 4等位基因的频率为 2 2 6 % ;突变频率在糖尿病伴有肥胖者与糖尿病体重正常者之间差异有显著性。突变与体质指数、收缩压、舒张压及HDL C相比有统计学意义。结论 糖尿病患者 β3 肾上腺素能受体基因突变与肥胖可能有关。

Objective To investigate the association between the mutation of β 3-adrenergoc receptor gene and obesity in patients with type 2 diabetes. Methods Body mass, waist-hip ratio, blood pressure and blood lipids were measured in 154 type 2 diabetic patients. Polymerase chain reaction and the restriction fragment length polymorphism analysis were used to determine the wild, heterozygous and homozygous forms of β 3-adrenergoc receptor gene. Results The frequency of the Trp64Arg mutation was 42.5% and the frequency of Arg64 allele was 22.6%. The mutation frequency of the genetic types was significantly different between the obese and non-obese type 2 diabetes mellitus patients. The body mass, systolic blood pressure, diastolic blood pressure, HDL-cholesterol were significantly different, when those with Trp64Arg heterozygous were compared with those with Trp64 homozygous.Conclusions The genetic mutation of β 3-adrenergoc receptor in patients with type 2 diabetes is probably related to obesity.