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人胆囊上皮细胞的分离、培养及鉴定 被引量:6

Isolation, culture and identification of human gallbladder epithelial cells
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摘要 目的 探索人胆囊上皮细胞的较佳分离方法和合适的生长条件 ,为研究胆囊的生理功能和相关疾病打下基础。方法采用IV型胶原酶及钝性擦刮法分离细胞 ,IV型胶原包被培养皿 ,DMEM/HamF12培养基联合培养。结果 每个胆囊可分离得到( 5~ 10 )× 10 7个胆囊上皮细胞 ,接种 1周内生长旺盛 ,第 3、4天达到峰值 ,呈现扁平多角形成片贴壁状态。结论 IV型胶原酶消化法可成功分离人胆囊上皮细胞 ,DMEM/HamF12培养基可提供较为满意的生长条件。 Objective To explore the suitable method for separation and growth conditions of human gallbladder epithelial cells (HGBEC) for establishing the basis of research work in physiological function of gallbladder and its related diseases. Methods HGBEC were isolated with collagenase type IV and cultured in DMEM/Ham F12 medium. The cultural dishes were firstly coated with collagen type IV and bovine calf serum. Morphological changes of the cells were observed and the growth curve of the cells was made. Results A total of (5-10)×10 7 cells were obtained from a single human gallbladder. These cells grew vigorously within one week and reached the maximum at the 3rd and the 4th day. The cells were flat and multangular in shape, and attached to the dishes. Conclusion Collagenase type IV digestion can successfully separate the HGBEC, and DMEM/Ham F12 can provide satisfactory growth condition for HGBEC.
出处 《第三军医大学学报》 CAS CSCD 北大核心 2001年第9期1109-1111,共3页 Journal of Third Military Medical University
基金 国家自然科学基金资助项目 (3 9970 0 3 9)
关键词 胆囊上皮细胞 Ⅳ型胶原酶 Ⅳ型胶原 细胞培养 gallbladder epithelial cells collagenases Collagen
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  • 1蔡文琴,实用免疫细胞化学与核酸分子杂交技术,1994年,496页

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  • 1胡向农,张忠林,杨建军.大鼠附睾上皮细胞的体外培养与研究[J].南京铁道医学院学报,1999,18(1):1-3. 被引量:4
  • 2Oda D, Lee S P, Hayashi A. Long term culture and Partial charactarization of dog gallbladder epithelial cells [ J ]. Lab Invest, 1991, 64 (5): 682 - 892.
  • 3Joplin R. Effect of EGF on bile duct epithelial cells[J]. Investigation,1992, 90(7): 1284 - 1289.
  • 4Dalei Shao, Shamina M, Rangwala, et al. Interdomain communication regulating ligand binding by PPAR-γ.Nature,1998;396(26):377.
  • 5Bone RC, Balk RA, Cerra FB, et al. Definitions for sepsis and organ failare and guidelines for the use of innovative therapies in sepsis. Chest,1992;10(6):1644.
  • 6Michie HR, Manogue KR, Spriggs DR, et al. IL-1β induced by TNF-α in macrophages in vivo. New Engl J Med,1998;318(5):1481.
  • 7Fitzegerald DJ, Cecere G. Hemofiltration and inflammatory mediators. Perfusion,2002;17(Supp1):23.
  • 8Hsueh WA, Jackson S, Law RE, et al. Control of vascular cell proliferation and migration by PPAR-gamma: a new approach to the macrovascularications of diabetes. Diabetes Care,2001;24(2):392.
  • 9蔡文玲 王伯沄.实用免疫细胞化学与核酸分子杂交技术[M].成都:四川科学技术出版社,1994.496-497.
  • 10若景森 韩文胜 卓健生 卢云 焦兴元.杨毅军细菌L型在胆囊结石形成中作用的探讨[J].世界华人消化杂志,1999,7:170-170.

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