摘要
目的 研究大蒜素对人肝癌细胞中VEGFmRNA表达的影响。方法 应用逆转录聚合酶链反应 (RT PCR)方法。以HPRT (hypoxanthinephosphoribosyltransferase)为内标。结果 大蒜素有明显抑制人肝癌细胞VEGFmRNA表达的作用 ,与对照组相比 ,其VEGFmRNA表达水平降低了约 66 3 6% (P <0 0 5 )。结论 大蒜素是人肝癌细胞表达VEGF基因的调节剂 ,能抑制人肝癌细胞VEGF基因的表达。
AIM To investigate the effect of allicin on the regulation of VEGF mRNA expression in human hepatocellular carcinoma cells. METHODS Hepatocellular carcinoma cells were treated with the concentration 10 μg·L -1 allicin in culture medium,and then the relative VEGF mRNA level at 8 h in human hepatocellular carcinoma cells was evaluated by reverse transcriptase polymerase chain reaction using HPRT(hypoxanthine phosphoribosyltransferase)as an internal control standard. RESULTS The expression of VEGF gene mRNA was inhibited obviously by allicin. Compared with control group, the relative expression level of VEGF gene mRNA was decreased by about 66 36%( P <0 05). CONCLUSION Allicin is the regulator of VEGF gene expression, it can inhibit VEGF gene expression in human heptoma cells.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2001年第5期531-533,共3页
Chinese Pharmacological Bulletin
关键词
大蒜素
表皮生长因子
表皮生长因子受体
肝细胞癌
MRNA
phosphorothioate oligodeoxynucleotides
hepatocellular carcinoma cells
reverse transcriptase polymerase chain reaction
vascular endothelial growth factor
allicin