摘要
本文选用^(126)Ⅰ-UdR进行肿瘤靶细胞同位素标记,并用于LAK细胞活性测定。靶细胞^(125)Ⅰ-UdR标记率在一定时间内随标记时间延长而增加,与^(125)Ⅰ-UdR和FUdR的剂量呈正相关关系,其自发释放随效靶作用时间的延长而增加.将此方法用于LAK活性测定取得较好的实验结果,并具有外照射损伤小、标记率高、自发释放相对低而特异性释放高等优点。
This paper reported the labelling of tumor target cell with 125I-UdR isotope and its use in detecting the LAK cell activity. The results showed that the longer the labelling time in a period time and the higher the 125I-UdR and/or FUdR concentration, the higher, the 125I-UdR labelling rate of tumor target cells. The spontaneous release was increasing with the effector and target reaction time. We dected the Lymphokine-activated killer (LAK) cell activity using this method and got better results. In addition, there were a lot of advantages such as lower spontaneous release, higher labelling rate and specific release, etc.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1991年第1期50-53,共4页
Immunological Journal
关键词
LAK细胞
肿瘤细胞
碘125
胸苷
标记
125I-UdR isotope, Lymphokine-activated killer (LAK) cell, Tumor cell