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肿瘤靶细胞^(125)Ⅰ-UdR标记方法的建立及其在LAK活性测定中的应用 被引量:2

Labelling of Tumor Target Cells with ^(125)I-UdR Isotope and its Application to Detection of LAK Cell Activity
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摘要 本文选用^(126)Ⅰ-UdR进行肿瘤靶细胞同位素标记,并用于LAK细胞活性测定。靶细胞^(125)Ⅰ-UdR标记率在一定时间内随标记时间延长而增加,与^(125)Ⅰ-UdR和FUdR的剂量呈正相关关系,其自发释放随效靶作用时间的延长而增加.将此方法用于LAK活性测定取得较好的实验结果,并具有外照射损伤小、标记率高、自发释放相对低而特异性释放高等优点。 This paper reported the labelling of tumor target cell with 125I-UdR isotope and its use in detecting the LAK cell activity. The results showed that the longer the labelling time in a period time and the higher the 125I-UdR and/or FUdR concentration, the higher, the 125I-UdR labelling rate of tumor target cells. The spontaneous release was increasing with the effector and target reaction time. We dected the Lymphokine-activated killer (LAK) cell activity using this method and got better results. In addition, there were a lot of advantages such as lower spontaneous release, higher labelling rate and specific release, etc.
出处 《免疫学杂志》 CAS CSCD 北大核心 1991年第1期50-53,共4页 Immunological Journal
关键词 LAK细胞 肿瘤细胞 碘125 胸苷 标记 125I-UdR isotope, Lymphokine-activated killer (LAK) cell, Tumor cell
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  • 1陈慰峰,庞学文,张惠华,张孙曦,白俊海.Ⅰ—UdR释放试验测定杀伤T细胞的细胞毒作用[J]中国免疫学杂志,1985(04).
  • 2于永利,杨贵贞.人参花总皂甙在体外对小鼠NK活性的影响[J]中国免疫学杂志,1985(02).
  • 3Stephen E. Ettinghausen,Steven A. Rosenberg. The adoptive immunotherapy of cancer using lymphokine activated killer cells and recombinant interleukin-2[J] 1986,Springer Seminars in Immunopathology(1):51~71

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