骨髓增生异常综合征的粒-单核祖细胞初步研究

PRIMARY INVESTIGETION OF GRANULOCYTE MACROPHAGE PROGENITOR CELL (CFU-GM) IN MYELODYSPLASTIC SYNDROMES

用血浆凝块法检查23例骨髓增生异常综合征(MDS)的骨髓粒-单核祖细胞(CFU-GM)体外生长特点,并与正常骨髓进行比较。MDS的CFU-GM集落产率明显低于正常,且从RA→RAEB→RAEBt逐渐减少;集簇/集落比值明显增高,并有逐渐增高趋势。结果表明,MDS是造血干(祖)细胞缺陷所致的克隆性疾病。认为,骨髓CFU-GM的培养对MDS的诊断、分型及与其它骨髓增生性疾病鉴别有一定意义。

The generating features of CFU-GM in 23 cases of myelodysplastic syndromes (MDS) were researched in vitro by plasma-coagulated method and compared with controls. The colony forming efficiency of CFU-GM was obviously lower in MDS than controls, and the ratio of cluster to colony was significantly increasing. Among different types of MDS, the colony forming rate from RA to RAEB and RAEBT was gradually decreased and the ratio of cluster to colony tended to increase progressively.The result indicated that MDS was clonal hemopathy of hemopoietic stem cell disorders. Determination of CFU-GM is useful for diagnosis and classification of MDS and differentiation from other myeloproliferative diseases.