小鼠脾保留和自体移植脾组织抗疟原虫感染的超微结构动态观察

DYNAMIC ULTRASTRUCTURAL OBSERVATION OF PRESERVED SPLEEN AND TISSUE OF AUTOLOGOUS SPLENIC TRANSPLANTATION IN ANTIPLASMODIUM INFECTION

目的探讨脾脏在急性疟原虫感染中所发挥的作用和移植脾组织的结构和功能。方法 90只昆明株小鼠分为假手术组、半脾保留组和半脾移植组。手术后 3个月经疟原虫感染后动态进行组织学观察。结果各组脾脏病理变化基本一致 ,感染中期脾脏红髓内分化增殖的网状细胞与网状纤维连接成网状支架 ,和被其包围的各种血细胞共同构成血脾屏障 ,从而保护了机体。结论在疟原虫作用下 ,移植脾组织与保留脾组织变化近似 。

ObjectiveTo explore the function and morphology of preserved spleen and tissue of autologous splenic transplantation in antiplasmodium infection.MethodsA total of 90 mice were divided into three groups:①sham operation. ②hemisplenectomy. ③hemisplenic autotransplantation. Three months after operation plasmodial parasites were inoculated. Histological changes in spleen tissue of 3 groups were examined by LM, TEM and SEM.ResultsThe pathological changes of spleen tissue in 3 groups were similar. In the middle infective phase,the reticulocyte of red pulps proliferated and connected with reticular fibers, and a blood spleen barrier was formed. ConclusionThe tissue of transplanted spleen is similar to that of the preserved spleen and may provide the similar protective function.