贯叶金丝桃愈伤组织的诱导及其有效成分的定性

Induction of Callus of Hypericum perforatum L. and Qualitative Identification of Active Constituents of its Callus

目的 :诱导出贯叶金丝桃的愈伤组织 ,并确定其中含有金丝桃素。方法 :在不同条件下采用组织培养技术 ,进行贯叶金丝桃的愈伤组织诱导研究 ,用HPLC方法确定其中含有的金丝桃素。结果和结论 :最佳诱导条件是MS基本培养基加入生长调节物质 2 ,4 D(4 μg·L-1)和 6 BA(0 .2 μg·L-1)。叶腋等分生组织较多的部位易于诱导愈伤组织。诱导出的愈伤组织 。

Objective: To induce the callus of H. perforatum and identify hypericin and pseudohypericin of its callus. Method: The callus was induced in different culture conditions , and active constituents were determined by HPLC. Result and conclusion: The inductions of callus from different parts were discussed, the induction rate of the leaf axil being the highest. The MS basic medium with 4 μg·L -1 2,4 D and 0.2 μg·L -1 6 BA was the best of all screened media. Hypericin in the callus is determined by HPLC.