异种(猪)骨基质明胶修复兔桡骨缺损模型的实验研究

The Experimental Study of the Heterologous Bone Matrix Gelatin (pBMG) for Repair of the Radius Segmental Defect of Rabbits

目的 :通过建立兔桡骨骨缺损模型 ,研究异种 (猪 )骨基质明胶 (pBMG)与单纯骨传导物质在促进骨愈合上的差别。方法 :把 16只新西兰大白兔的双侧桡骨制造成 10mm的骨缺损模型 ,分别植入pBMG、自体骨段和羟基磷灰石 (HAC)。在术后即刻 ,2周 ,4周和 8周进行放射学 ,组织学 ,扫描电镜和骨密度的检测。结果 :发现pBMG组术后 4周即有新骨形成 ,到 8周缺损已愈合 ;组织学发现植入 pBMG的缺损处为多中心成骨 ,到术后 8周已基本吸收 ,而植入HAC的骨缺损处也可见到缺损愈合 ,但HAC没有降解。结论 :从实验结果可以发现异种骨基质明胶是一种具有好的骨诱导活性和生物降解性的治疗骨缺损的填充材料。

Objective: To find out the difference of bone heal effect between pBMG and HAC. Methods: Ten millimeters long segmental defect was created in the bilateral radius in sixteen New Zealand white rabbits. These defects were embedded with pBMG, autologous bone and hydroxyapatite cement (HAC), and examined by radiographic, histological, scan electron microscope (SEM) and bone minal dense (BMD) within 8 weeks. Results: There are obvious bone formation at the defect site at four week after operation and when at 8 weeks, the defect was repaired. Conclusion: The effect of BMG is similar to that of autologous bone and prior to the effect of HAC.