BALB/C小鼠Hp感染胃炎动物模型的建立和免疫治疗实验研究

Establishment of BALB/C Mouse Model with Hp Infection and Experimental Research on Its Immunization Therapy

目的 通过人类Hp的接种 ,建立BALB/C小鼠感染胃炎动物模型 ,研究纯化尿素酶在治疗H .pylori(Hp) ,感染中的作用。方法 取小鼠胃粘膜组织 ,通过Giemsa染色和HE染色评价SS1在BALB/C小鼠的定植及病理组织学改变。结果 4、8周时小鼠胃窦、胃体可见较少量的Hp定植 ,16周后B、C、D组Hp定植量明显增多 ;4周时动物未发现有明显炎症反应 ,第 8周时小鼠腺胃出现一定程度的炎症反应 ,16周时B、C、D组小鼠胃窦及胃体的炎症明显加重。经免疫治疗各组Hp根除情况如下 ,尿素酶 +霍乱毒素 (CT) +羟磷石灰 (HAP)为 61.5 3 % (8/ 13 ) ,而单纯尿素酶、CT +HAP根除率为零。结论 Hp可感染BALB/C小鼠导致胃炎发生 ,其病理改变与人类类似 ,另外由尿素酶加免疫佐剂组成的口服免疫治疗 ,有根除已感染的Hp的作用。

Objectives To establish a model of BALB/C mouse infected by Hp and to investigate the effects of the purified urease in the treatment of Hp infection.Method The stomach biopsies of all mice were collected to assess the colonization of Hp and their histological changes by HE staining,Giemsa staining.Results In 4,8 weeks,Hp were clearly visible at gastric antrum and body.The number of Hp colonization increased in B,C,D groups respectively in 16 weeks.The animals had no inflammatory changes in 4 weeks.In 16 weeks more serious gastritis was observed in B,C,D groups.After immunization therapy,Hp were eradicated in 61.5%(8/13) mice treated with urease plus cholera toxin(CT).No therapeutic effects were found in the groups with urease or CT alone.Conclusions Hp can colonize in the stomach mucosa of BALB/C and lead to chronic active gastritis,whose pathological changes are similar to human's.The oral immunization treatment consisting of urease and mucosal adjuvant CT is effective in the eradication of Hp infection.