以基因枪法将Bt杀虫蛋白基因导入常规玉米自交系的研究

Studies on Transferring Insecticidal Protein Gene from Bacillus Thuringiensis into Excellent Maize Inbred-line via Microprojectile Bombardment

以生产上大面积使用的优良玉米自交系 792 2为材料 ,以来源于其幼胚的Ⅱ型胚性愈伤为受体 ,经预实验选择标准制弹用量一半的金粉 ,通过基因枪轰击法 ,将Bt杀虫蛋白基因转入其细胞中 ,并再生了大量的转基因植株。抗性植株再生频率为 78 40 % ,PCR分子检测证明目的基因已转入玉米中 ,转化频率为 3 0 9%。

Using maize inbred-line 7922 used largely in agriculture production as material and typeⅡ embryogenic calli from their immature embryos as acceptor,the insecticidal protein from Bacillus thuringiensis was transformed into the cells via Microprojectile Bombardment.Half of the gold particles according to statard protocol were applied after pre-experiment.A large number of resistant plants had been regenerated and regeneration frequency reached to 78.40%.After PCr analysis,it was proved that the target Bt gene had been integrated into regenerated plants.The transformation frequency was as high as 3.09%.