钙调神经磷酸酶在哮喘豚鼠气道重塑中的作用

Role of calcineurin in airway remodeling in guinea pig model of asthma

目的 :观察钙调神经磷酸酶 (CaN)在哮喘豚鼠气道重塑中的作用。方法 :实验分 3组 :对照组、哮喘组及CaN抑制剂环孢霉素 (CsA)组 ,测定指标包括 :①支气管肺泡灌洗液 (BALF)蛋白含量、细胞计数及分类 ;②大气道平滑肌 [3H] TdR掺入量 ;③肺组织切片中小气道壁厚度及气道平滑肌厚度 ;④气管和肺组织CaN活性。结果 :①BALF :CsA组蛋白含量、细胞计数及嗜酸粒细胞分别较哮喘组少 4 6%、5 1%及 60 % (P <0 0 1) ;②大气道平滑肌 [3H] TdR掺入量 :CsA组较哮喘组低 2 2 % (P <0 0 5 ) ;③小气道壁厚度 :CsA组较哮喘组少 3 4 % (P <0 0 1) ;气道平滑肌厚度 :CsA组较哮喘组少 3 7% (P <0 0 1) ;④肺组织CaN活性 :CsA组较哮喘组低 5 2 % (P <0 0 1) ;气管CaN活性 :CsA组较哮喘组低 4 4 % (P <0 0 1)。结论 :CsA可减轻哮喘豚鼠气道重塑 。

AIM: To investigate the role of calcineurin (CaN) in airway remodeling in guinea pig model of asthma.METHODS: Male guinea pigs were randomly divided into three groups: control, asthma group and CsA group. The following parameters were measured: 1. The protein content, cell count and differential count of BALF; 2. The amount of TdR incorporation into central airway smooth muscle; 3. The mean thickness of airway wall and airway smooth muscle of small airwaysl; 4.CaN activity of trachea and lung tissue.RESULTS: 1. The protein content, cell count and eosinophil of BALF in CsA group were 46%, 51% and 60% lower than those in asthma group, respectively ( P <0 01); 2. TdR incorporation in CsA group was 22% lower than that in asthma group ( P <0 05);3. The mean thickness of airway wall and airway smooth muscle were 34% and 37% less in CsA group than those in asthma group, respectively ( P <0 01); 4. CaN activity of lung tissue and trachea were 52% and 44% lower in CsA group than those in asthma group, respectively ( P <0 01).CONCLUSION: CsA reduced airway remodeling in guinea pig model of asthma, indicating the role of CaN in the airway remodeling.