摘要
本文根据Steinman方法并稍加改良从小鼠脾脏分离树突状细胞获得成功。经抗小鼠树突状细胞单克隆抗体(33D_1)作间接免疫荧光染色、微量细胞毒试验及EA花环形成试验等方法鉴定,其纯度大于80%。且经透射和扫描电镜观察,其形态结构明显不同于巨噬细胞。
Splenic dendritic cells were isolated from mouse, using the method of Steinman with minor modifications. These isolates were identified by indirect immunofluorescen-ce, applying McAb 33D1, complement dependent cytotoxicity and EA rosettes forming test. The purifity was greater than 80%. Electron microscopic studies on the dendritic cells showed distinctive morphologic features other than those of macrophages.
出处
《上海免疫学杂志》
CSCD
北大核心
1991年第3期129-132,共4页
Shanghai Journal of Immunology