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小鼠脾脏树突状细胞的分离与鉴定 被引量:19

Isolation and Identification of Mouse Spleen Dendritic cells
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摘要 本文根据Steinman方法并稍加改良从小鼠脾脏分离树突状细胞获得成功。经抗小鼠树突状细胞单克隆抗体(33D_1)作间接免疫荧光染色、微量细胞毒试验及EA花环形成试验等方法鉴定,其纯度大于80%。且经透射和扫描电镜观察,其形态结构明显不同于巨噬细胞。 Splenic dendritic cells were isolated from mouse, using the method of Steinman with minor modifications. These isolates were identified by indirect immunofluorescen-ce, applying McAb 33D1, complement dependent cytotoxicity and EA rosettes forming test. The purifity was greater than 80%. Electron microscopic studies on the dendritic cells showed distinctive morphologic features other than those of macrophages.
机构地区 镇江医学院
出处 《上海免疫学杂志》 CSCD 北大核心 1991年第3期129-132,共4页 Shanghai Journal of Immunology
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  • 1刘津红,郑武飞.小鼠脾脏树突状细胞的制备与鉴定[J]中国免疫学杂志,1989(06).

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