摘要
目的 :研究高效液相色谱法测定山药中尿囊素的含量。方法 :用反相高效液相色谱法 ,分析柱采用ZorbaxODX柱(2 5 0mm× 0 .4mm) ,分析流动相为甲醇 氯仿 水 (0 .5∶0 .12∶10 0 ) ,流速 0 .5ml·min-1,检测波长为 2 2 4nm。结果 :尿囊素浓度在 0 .0 2~ 0 .10 μg范围内线性良好 (r=0 .9998) ,相对平均回收率为 99.5 7%,日内精密度小于 1.74%。结论 :方法简便、快速、准确、可行。
OBJECTIVE To establish the HPLC method for determination of allantoin in Rhizoma Dioscoreae. METHODS The method of RP-HPLC was used.The analytical Column(250mm× 4.6mm) was packed with Zorbax ODS. The Mobile phase consists of Methanol-chloroform-water( 0.5∶ 0.12∶100,v/v) at a flow rate of 0.5ml·min -1,the ultraviolet wavelength was set at 224nm. RESULTS Linear range of Allantoin was 0.02~ 0.10μg (r= 0.9998).The relative recovery was 99.57%,and the within day RSD less than 1.74%. CONCLUSIONS The method is simple, rapid, sensitive and accurate.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2001年第7期413-414,共2页
Chinese Journal of Hospital Pharmacy