摘要
本文对葡萄糖氧化酶产生菌Z-I-C的分批发酵与恒化培养进行了研究。实验发现,在以葡萄糖为生长限制性基质的恒化培养中,该产生菌的维持系数为0.04g葡萄糖/g菌体·h,生长得率系数Y_G^(max)=0.714 g菌体/g葡萄糖,最大比生长速率μ_(max)=0.385h^(-1),饱和常数K_2=4.76g/L,理论最适稀释度D_m=0.260h^(-1),最大酶比活(E/X)_(max)为2.16×10~3u/mg,其值较分批发酵的最大酶比活(1.51×10~3u/mg)提高43%。当向恒化培养的补料培养基中添加0.02%的α-甲基-D-葡萄糖时,由于该葡萄糖结构类似物的诱导作用,可使(E/X)_(max)达3.11×10~3u/mg,较分批发酵之值提高106%。
The biosynthesis of glucose oxidase(GOD) by an unidentified Penic-illium strain Z-I-C in batch and chemostat culture was studied. In aglucose-limited chemostat culture, the experimental results showed thatthe maintenance coefficient of this Strain was 0.04g glucose/g dry cellWt·h; yield factor of growth was 0.714 g dry cell wt./g glucose; maxi-mum value of specific growth rate was 0.385h^(-1) and K: was 4.76g/L.Optimum dilution rate in theory was 0.260h^(-1). (E/X)^(max) was increasedfrom 1.51×10~3 u/mg in batch culture to 2.16×10~3 u/mg in chemostat cu-lture and to 3.11×10~3 u/mg in chemostat culture supplemented with0.02% inducer, α-methyl-D-glucose, an increase of 43% or 106% comp-ared with that of batch culture, respectively.
出处
《生物工程学报》
CAS
CSCD
北大核心
1991年第4期351-358,共8页
Chinese Journal of Biotechnology