摘要
目的 :观察清肝活血方对酒精性肝病大鼠肝组织 型胶原表达的影响。方法 :4 5只大鼠用乙醇、玉米油、吡唑等制备酒精性肝病大鼠模型 ,随机均分为模型组 ,清肝活血方低、高剂量组 ,小柴胡冲剂组 ,用免疫组化观察肝组织 型胶原的分布 ,RT- PCR法检测大鼠肝组织 型胶原 m RNA表达 ,并与对照组比较。结果 :酒精性肝病大鼠模型表现为肝功能异常 ,以门冬氨酸氨基转移酶 (AST)变化为显著 ,模型组大鼠肝组织在汇管区有少量 型胶原的分布 ,部分向小叶内延伸 ;正常组大鼠几乎无 型胶原 m RNA表达 ,模型组有明显表达 ,清肝活血方高剂量组 型胶原及其 m RNAR的表达均明显下降 (P <0 .0 5 )。结论 :清肝活血方能有效预防酒精引起的肝损伤、肝纤维化的发生、发展 ,其机制可能与其抑制 型胶原 m
Objective: To observe the effect of Qinggan Huoxue decoction on the expression of the liver collagen type I of wistar rats with alcoholic liver disease (ALD). Methods:Forty five wistar rats with ALD induced by alcohol, corn oil and pyrrole were randomly divided into four groups: model group, Xiaocaihu granule group, low dose group and high dose group of Qinggan Huoxue decoction. The distribution of liver collagen type I (CI) was detected by using immunohistochemical technique and the expression of liver CI mRNA was measured by RT PCR technique. Results:The hepatic function of the model group was abnormal, especially AST. A small quantity of collagen type I distributed in portal area and partially extended into hepatic lobule. In normal group there was no expression of CI mRNA and noticeable expression in model group. While in higher dose group of Qinggan Huoxue decoction CI and the expression of CI mRNA were significantly decreased (P<0.05).Conclusion: Qinggan Huoxue decoction could effectively prevent the generation and development of liver injury and fibrosis induced by alcohol. The mechanism may be related with its function of restraining the expression of CI mRNA.
出处
《中国中西医结合消化杂志》
CAS
2002年第1期24-26,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
上海市青年科技启明星培养计划资助项目 (No.99QB14 0 0 4 )
关键词
酒精性肝炎
清肝活血方
Ⅰ型胶原
alcoholic liver disease
Qinggan Huoxue decoction
collagen type I