AFP和AFP异质体对405例原发性肝癌的诊断价值探讨

The value of combine determination of AFP variants and AFP in the diagnosis of primary liver cancer

目的 探讨甲胎蛋白 (AFP)和甲胎蛋白异质体 (AFPVariants ,AFP V)对原发性肝癌 (PHC)的诊断价值。方法 用酶联免疫吸附试验 (ELISA)和交叉免疫电泳、放射性自显影法分别检测AFP和小扁豆凝集素亲和性甲胎蛋白异质体 (LCA AFP V)的血清含量。结果 4 0 5例PHC患者中 ,AFP阳性占 75 .8% ,LCA AFP V阳性占 5 4 .3% ;2 10例AFP低浓度阳性患者中 ,LCA AFP V阳性 12 5例 ,而 97例AFP高浓度阳性患者 ,有 95例伴有异质体的升高 ;98例AFP阴性患者中 ,35例LCA AFP V阳性 ;2 2 3例非PHC中AFP阳性占 4 2 .2 % ,LCA AFP V阳性占 2 5 .6 %。结论 单独应用AFP或AFP V均有一定的局限性 。

Objective To investigate the value of combine determination of AFP and AFP variants in the diagnosis of primary hepatic cancer(PHC). Methods Serum AFP and LCA affinited AFP were determined in 641 patients suspected suffering from PHC by ELISA and immune affinity electrophoresis. Results 220(54.3%) patients are LCA affinited AFP positive and 307(75.8%) patients are AFP positive in 405 PHC. 125 patients are LCA affinited AFP positive in 210 patients with low concentration AFP positive, whereas 95 patients are LCA affinited AFP positive in 97 patients with high concentration AFP positive. On the other hand, we have fonnd 35 patients LCA affinited AFP positive in 98 AFP negative patients. In 223 non primary liver cancer patients, the APF positive rate was 42.2% and LCA affinited AFP positive 25.6%. Conclusion Combine determination of AFP variants and AFP will improve the diagnosis of PHC especially in low concentration AFP positive patients.