HPLC测定贯叶金丝桃中黄酮的含量(英文)

DETERMINATION OF FLAVONOIDS IN HYPERICUM PERFORATUM BY HPLC ANALYSIS

目的 建立同时测定贯叶金丝桃中 4种黄酮芦丁、金丝桃苷、扁蓄苷和槲皮素的HPLC分析方法。方法 C18柱 ;流动相A :水 (磷酸调pH 3 1～ 3 5) ,B :乙腈 ,梯度洗脱 ;流速 1 0mL·min- 1;检测波长2 54nm。结果 线性范围为芦丁 1 376～ 8 2 56μg·mL- 1(γ=0 9999) ,金丝桃苷 3 1 60～1 8 960 μg·mL- 1(γ=0 9996) ,扁蓄苷 0 968～ 5 80 8μg·mL- 1(γ =0 9998) ,槲皮素 0 776～ 4 656μg·mL- 1(γ =0 9993)。平均加样回收率为芦丁 97 8% ,RSD(n=3) 4 8% ;金丝桃苷 1 0 0 7% ,RSD(n=3) 4 1 % ;扁蓄苷 97 3% ,RSD(n =3)0 7% ;槲皮素 1 0 0 5 % ,RSD(n =3) 4 4%。 4个化合物的精密度RSD(n =5)均 <2 % ,重现性RSD(n =5)均<3 %。结论 本方法简单、有效、可行 。

AIM To establish an HPLC method for simultaneous determination of four flavonoids in Hypericum perforatum L., ruitn, hyperin, avicularin and quercetin. METHODS C 18 column was used, the chromatography was carried out with a linear gradient program. The mobile phase was A: H 2O (pH 3 0～3 5 adjusted with phosphoric acid) and B: acetonitrile, at flow rate of 1 0 mL·min -1 , peaks were detected at 254 nm. RESULTS The linear range of rutin was 1 376～8 256 μg·mL -1 (γ=0 9999), hyperin 3 160～18 960 μg·mL -1 (γ=0 9996), avicularin 0 968～5 808 μg·mL -1 (γ=0 9998) and quercetin 0 776～4 656 μg·mL -1 (γ=0 9993). The average recovery of rutin was 97 8%, RSD 4 8% ( n =3), hyperin 100 7%, RSD 3 7% ( n =3), avicularin 97 3%, RSD 0 8% ( n =3) and quercetin 100 5%, RSD 4 4% ( n =3). All of RSDs of precision were less than 2% ( n =5), reproducibilities less than 3%. CONCLUSION The method is simple, effective and feasible. It can be used to determine flavonodis in Hypericum perforatum .