重组人角质细胞生长因子-2治疗大鼠角膜碱烧伤的实验研究

Experimental study on recombination human keratinocyte growth factor-2 in treating corneal alkaline burn in rats

目的观察重组人角质细胞生长因子-2(recombination human keratinocyte growth factor-2,rhKGF-2)对实验性大鼠角膜碱烧伤的治疗效果。方法 70只雌性SD大鼠用1 mol/L Na OH溶液进行角膜碱烧伤后,随机分为模型对照组、重组牛碱性成纤维细胞生长因子滴眼液组(recombinant bovine basic fibroblast growth factor in eye drop,bFGF组)、rhKGF-2低剂量组、rhKGF-2中剂量组和rhKGF-2高剂量组,14只/组,bFGF组给予4 500 IU/ml的bFGF,rhKGF-2各剂量组分别给予12. 5、25、50μg/ml的rhKGF-2,50μl/次,3次/d,连续给药14 d。于治疗第1 d、第3 d、第7 d和第14 d,进行角膜损伤程度评级和角膜内皮细胞愈合率测定,于治疗第7 d和第14 d,进行体重和角膜组织病理形态学观察。结果各时点各组大鼠体重、角膜损伤程度差异无统计学意义(P>0. 05); bFGF组大鼠治疗14 d角膜厚度降低(P<0. 05); rhKGF-2组大鼠治疗后第3 d、第7 d内皮细胞愈合率增加(P<0. 05),治疗14 d角膜厚度降低(P<0. 05);与bFGF组大鼠比较,rhKGF-2高剂量组大鼠治疗3 d的内皮细胞愈合率增加(P<0. 05)。结论 rhKGF-2对大鼠角膜碱烧伤模型具有治疗作用,早期治疗效果明显。

Objective To study the therapeutic effect of recombination human keratinocyte growth factor-2 (rhKGF-2) on experimental corneal alkaline burn in rats. Methods Corneal injuries were induced by alkaline burn with 1 mol/L NaOH solution in 70 female SD rats. The rats were then randomly divided into the model control group, the recombinant bovine basic fibroblast growth factor in eye drop (bFGF) group and the low-, middle- and high-dose rhKGF-2groups (each n=14). The bFGF group was treated with 4,500 IU/mL bFGF, and the low-, middle- and high-dose rhKGF-2 groups were respectively treated with 1.25 μg/ml, 25 μg/ml and 50 μg/ml rhKGF-2, 50 μl per time, 3 times a day for 14 days. The corneal injury grades and the healing rate of corneal endothelium were measured on the 1st, 3rd, 7th and 14th days after the start of treatment. Body weight and corneal histopathologic changes were examined on the 7th and 14th days after the start of treatment. Results No statistically significant differences were found in body weight and corneal injury degree among all groups on each time points (P>0.05). Corneal thickness of the rats in the bFGF group decreased on the 14th day after the start of treatment (P<0.05). Corneal endothelium healing rate of the rats in the rhKGF-2 groups increased on the 3rd and 7th days after the start of treatment (P<0.05), whereas their corneal thickness decreased on the 14th day after the start of treatment (P<0.05). Compared with the rats in the bFGF group, corneal endothelium healing rate of the high-dose rhKGF-2 group increased on the 3rd day after the start of treatment (P<0.05). Conclusions rhKGF-2 is effective on experimental corneal alkaline burn in rats, especially in the early treatment.