基于栅藻延迟发光的制天南星、胆南星药性研究

Study of medicinal properties of Arisaematis Rhizoma Preparatum and Arisaema Cum Bile based on delayed luminescence of Scenedesmus sp.

目的寻找表征制天南星、胆南星寒热药性的延迟发光指标。方法非等间隔测量制天南星煎煮后26d内其煎煮液加入栅藻中的延迟发光,验证自然延迟发光平均强度和激发延迟发光线性拟合斜率(k)的稳定性。生物指示剂法测量6个批次制天南星、胆南星的延迟发光,高效液相色谱法测定制天南星、胆南星中β-谷甾醇的含量及胆南星中胆红素和胆酸的含量,并探索k值与成分含量的相关性。结果制天南星煎煮后14 d内k值较自然延迟发光平均强度更稳定。6个批次的制天南星的k值均高于胆南星。6个批次的制天南星、胆南星中β-谷甾醇含量与k值呈负相关,且相关关系显著,胆南星中胆红素和胆酸的含量与胆南星k值呈不显著的负相关。结论 k值是反映制天南星、胆南星药性的科学指标,为研究中药药性提供了新的方向。

Objective To find delayed luminescence parameters that could characterize the cold and hot properties of Arisaematis Rhizoma Preparatum and Arisaema Cum Bile. Methods Delayed luminescence of Arisaematis Rhizoma Preparatum with addition of Scenedesmus sp. within 26 d after decoction was measured in unequal interval, with aim to verify the stability of the natural delayed luminescence average strength and the linear fitting slope value(k) of excitation delayed luminescence. The delayed luminescence of six batches of Arisaematis Rhizoma Preparatum and Arisaema Cum Bile was measured using biological indicator method, and the content of β-sitosterol in Arisaematis Rhizoma Preparatum and β-sitosterol, bilirubin, and cholic acid of Arisaema Cum Bile was determined using high performance liquid chromatograph(HPLC) to analyze the correlation of k value and the above components content of Arisaematis Rhizoma Preparatum and Arisaema Cum Bile. Results K value of excitation delayed luminescence within 14 d after decoction was steadier than natural delayed luminescence average strength, and k values of six batches of Arisaematis Rhizoma Preparatum were all higher than that of Arisaema Cum Bile. A significant negative correlation between β-sitosterol contents and k values of six batches of Arisaematis Rhizoma Preparatum and Arisaema Cum Bile was found, and no significant negative correlation between bilirubin and cholic acid contents and k values of Arisaema Cum Bile was found. Conclusion K value of excitation delayed luminescence could indicate the differences of medicinal properties of Arisaematis Rhizoma Preparatum and Arisaema Cum Bile, which provides a new method for the study of medicinal properties of Chinese materia medica.