基于灰色关联分析和功效组分的大黄药材质量评价

Quality Evaluation of Radix et Rhizoma Rhei Based on Gray Correlational Analysis and Functional Components

目的:本文通过灰色模式识别和功效组分对大黄药材质量进行综合评价,探索不同道地产区大黄药材的差异。方法:利用HPLC法测定大黄药材中大黄素、大黄酸、大黄酚、芦荟大黄素、大黄素甲醚、大黄酸苷、大黄素苷、大黄酚苷、芦荟大黄素苷、大黄素甲醚苷、番泻苷A、番泻苷B、儿茶素、没食子酸14种功效成分的含量;采用Python 2.7对与大黄传统功效密切相关的功效组分进行灰色关联分析。结果:不同产地大黄质量存在一定的差异,采集的25批大黄中甘肃天祝所产唐古特大黄"泻下攻积"质量最佳;四川雅江所产唐古特大黄"清热解毒"质量最佳;甘肃礼县所产掌叶大黄"逐瘀通经"的质量最佳。结论:模式识别在中药质量评价领域有着广阔的前景,且从中药的临床功效出发,在功效组分的层面上进行模式识别,能为建立更加完整和科学的中药质量评价体系提供一个新的思路。

Objective: To comprehensively evaluate the quality of Radix et Rhizoma Rhei based on gray correlationalanalysis and functional components, and to explore the difference of Radix et Rhizoma Rhei in different genuineproducing areas. Methods: HPLC was utilized to analyze 14 main compositions contained in the samples, includingemodin, rhein, chrysophanol, aloe-emodin, physcion,rheinoside,physcion glucoside,chrysophan,aloe-emodinglucoside,emodin methyl glycoside, sennoside, sennoside B, catechin and gallic acid. Then python 2.7 software wasemployed for gray correlation analysis of functional components closely related to the traditional efficacy of Radix et Rhizoma Rhei. Results: The qualities of Radix et Rhizoma Rhei grow in different areas were different. Tanggute Radix et Rhizoma Rhei grew in Tianzhu Gansu had the best effects of "expelling water retention and attacking the accumulation",and that grew in Yajiang Sichuan had the best effects of "clearing heat and removing toxin". Zhangye Radix et Rhizoma Rhei grew in Lixian Gansu had the best effect of"expelling stasis and unblocking the channels". Conclusion: Patternrecognition has broad prospects in the field of quality evaluation of traditional Chinese medicine. From the clinicalefficacy of traditional Chinese medicine, pattern recognition at the level of efficacy components can provide a new ideafor establishing a more complete and scientific quality evaluation system for traditional Chinese medicine.