大豆提取物对去卵巢大鼠脂代谢的作用

Effects of Soy Extract on Lipid Metabolism in Ovariectomized Rats

目的 :确定大豆提取物及其有效成分对去卵巢大鼠脂代谢的作用。方法 :Wistar大鼠 90只 ,随机分成正常组、假手术组、模型组、雌激素组、高剂量大豆黄酮组、低剂量大豆黄酮组、高剂量大豆提取物组、低剂量大豆提取物组、大豆多糖组 ,每组 10只。除假手术组与正常组外 ,其余各组均切除双侧卵巢。术后 1周开始喂养。 6周后处死动物 ,测定血清总胆固醇 (sTC)、血清甘油三酯 (sTG)、血低密度脂蛋白 (LDL)、肝组织中甘油三酯 (hTG)和胆固醇 (hTC)含量 ,取适量肝组织作病理学观察。结果 :切除卵巢后动物sTC ,LDL ,hTG ,hTC均有显著性增加 ,肝脏切片观察显示肝细胞中出现明显的脂滴。雌激素组与大豆提取物、黄酮组均表现不同程度降低去势大鼠sTC ,LDL ,hTG和hTC含量 ,但大豆多糖无作用 ,黄酮成分药理作用最强与雌激素相当。雌激素组肝脏切片有明显空泡样变性。结论 :大豆提取物有减弱去卵巢引起的病理改变 ,其有效成分为黄酮类化合物 ;雌激素虽有较强的替代卵巢作用 。

Objective:To study effects of soy extract on lipid metabolims in ovariectomized rats. Method: 90 Wistar rats were randomly divided into 9 groups:control group,sham group,model group,estrogen group,soy isoflavone group of high dose,soy isoflavone group of low dose,soy extract of high dose,soy extract of low dose,and soy polysaccharde group, 10 rats in each group. Except fer of control and sham groups,the test rats were ovariectomized. One week after operation,the rats were treated with different drugs. Six weeks after operation, the rats were killed, with serum and liver taken, and serumglycerol(sGT),cholesterol(sGC),LDL,HDL and liver homogenate hGT,hGC, measured. Result:The level of sGC,LDL in ovariectmized rats increased significantly,compared with that in control and sham groups. In liver both the level of hGT and hGC were higher than that in liver from control and sham groups. Administration of estrogen or soy extract or soy isoflavone could attenuate these in ovariectomized rats, but soy polysacchardes did not have any effects. Conclusion:Ovariectomized rats have an imbalance of lipid metabolism,the level of hGT and hGC were increased, and administration of estrogen,soy extracts or soy isoflavone could decrease these changes induced by ovariectomizing.