p53和C-Ha-ras基因在胃癌发生中的作用

The role of p53 and C-Ha-ras genes in gastrocarcinogenesis

目的 探讨 p5 3和C Ha ras基因突变在胃癌发生中的作用。 方法 用PCR SSCP和直接测序检测胃癌组织中C Ha ras(12、13密码子 )和 p5 3(外显子 7)基因的突变。 结果 胃癌组织中C Ha ras和 p5 3基因突变发生率显著高于浅表性胃炎和正常胃黏膜组织 ,基因的联合突变在部分胃癌组织中表达。结论 基因联合突变可能是胃癌发生的机制之一。

Objective To determine the role of p53 and C-Ha-ras gene mutation in the development of gastric cancer. Methods The point mutation of C-Ha-ras(codon12,13) and P53(Exon7) genes were detected by polymerase chain reaction -single strand conformation polymorphism analysis-direct sequence (PCR-SSCP-Sequence). Results The mutational rates of C-Ha-ras and P53genes in gastric cancer group were significantly higher than those in superficial gastritis group and normal group, the combinated mutation was detected in some gastric cancer patients. Conclusion The gene mutation of C-Ha-ras and p53 gene may play a role in gastric carcinogenesis.