细胞间粘附分子-1,2在肝内胆汁淤积孕鼠肝脏不同部位表达及其意义的研究

Study on the expressions of intercellular adhesion molecules-1,2(ICAM-1,ICAM-2) in the different sites of the liver of pregnant rats with ethinyl estradiol induced intrahepatic cholestasis.

目的 :研究细胞间粘附分子 1,2 (intercellularadhesionmolecules 1, 2 ,ICAM 1,2 )在肝内胆汁淤积 (intrahepaticcholestasis ,IHC)孕鼠肝脏不同部位的表达及其意义。方法 :应用雌二醇 (ethinylestradiol ,EE)诱导孕鼠IHC ,用流式细胞仪检测肝脏ICAM 1,2的表达。结果 :(1)用药第 5天 ,孕鼠血清丙氨酸转氨酶 (ALT)、天冬氨酸转氨酶 (AST)、总胆酸 (TBA)、碱性磷酸酶 (AKP)明显升高 ,肝脏组织学检查发现IHC ;(2 )孕鼠ICAM 1,2在肝细胞、肝门静脉内皮、肝胆管上皮的表达明显增高。结论 :粘附分子 (adhesionmolecules ,AM)表达异常可能是妊娠肝内胆汁淤积 (ICP)发病机理的一个重要环节。

Objective:To study on the expressions of intercellular adhesion molecules-1,2 (ICAM-1,ICAM-2) in the different sites of liver of pregnant rats with ethinyl estradiol induced intrahepatic cholestasis and their significance. Methods:The model of intrahepatic cholestasis of pregnant rats was induced by ethinyl estradiol, and then measured the expressions of ICAM-1,2 by flow cytometry(FCM). Results:(1)Serum ALT,AST,AKP and total bile acids of pregnant rats were significantly increased in fifth days after treatment with EE than that of without treatment with EE (P<0.05).Intrahepatic cholestasis had been discovered by microscopy.(2)The expressions of ICAM-1,2 on the cell of liver, hepatic vein endothelial cell and common bile duct endothelial cell were higher in the rats treatment with EE than that of without treatment with EE (P<0.05). Conclusion:Adhesion molecules may be an important link in the pathogenetic mechanism of ICP.