摘要
Callus was induced by embryos from mature seeds of Pinus serotina with MS medium contained 0.2 mg·L -1 of KT and 2.5 mg·L -1 of 2,4 D.After successive subculture and selection,six month old callus were transferred to a liquid medium with 0.2 mg·L -1 of KT and 0 5.0 mg·L -1 of 2,4 D and 200 mg·L -1 of LH for vibration culture,the suspension cultured cell lines were established with favorable dispersivity.A great amount of protoplasts were released from three month old cell suspension cultures,and protoplast yield and protoplast viability of suspension cells on linear phase are the highest under the enzyme combination cellulase R 10 2%+pectinase 0.25%?
Callus was induced by embryos from mature seeds of Pinus serotina with MS medium contained 0.2 mg·L -1 of KT and 2.5 mg·L -1 of 2,4 D.After successive subculture and selection,six month old callus were transferred to a liquid medium with 0.2 mg·L -1 of KT and 0 5.0 mg·L -1 of 2,4 D and 200 mg·L -1 of LH for vibration culture,the suspension cultured cell lines were established with favorable dispersivity.A great amount of protoplasts were released from three month old cell suspension cultures,and protoplast yield and protoplast viability of suspension cells on linear phase are the highest under the enzyme combination cellulase R 10 2%+pectinase 0.25%?
出处
《林业科学研究》
CSCD
北大核心
2002年第2期247-251,共5页
Forest Research
基金
国家林业局"亚热带林木培育重点实验室"基金项目 (1998~ 1999年 )
关键词
晚松
悬浮细胞系
原生质体
分离
Pinus serotina
cell suspension culture
protoplast isolation