鼻咽癌P53和c-erbB-2蛋白的表达与临床分期和复发的关系

Expression of P53 and c-erbB-2 protein in nasopharyngeal carcinoma and their correlation with the clinical staging and recurrence

目的 :探讨鼻咽癌 (NPC)P5 3、c -erbB - 2蛋白的表达与临床分期和复发的关系。方法 :搜集NPC蜡块标本 70例 ,应用免疫组化LSAB法 ,检测P5 3和c -erbB - 2基因蛋白的表达 ,将其表达结果与NPC临床分期和复发的关系进行研究分析。结果 :P5 3阳性表达率 6 8.5 7%,其与颈淋巴结转移与否无关 ,但P5 3表达阳性者 ,其转移之颈淋巴结多小于 4cm(P <0 .0 5 ) ;Ⅲ、Ⅳ期P5 3阳性表达率较Ⅰ、Ⅱ期高 (P <0 .0 5 ) ;P5 3的表达与放疗后鼻咽局部复发和远处转移无关。c -erbB - 2阳性率为 84 2 9%,与NPC临床分期和复发无相关性。结论 :P5 3蛋白在NPC病程的晚期表达较高 ,与转移之颈淋巴结大小有关。

Aim: To study the relationship between the clinical stage and recurrence and the expression of P53 and c-erbB-2 protein in nasopharyngeal carcinoma (NPC). Methods: The expression of P53 and c-erbB-2 in 70 cases of paraffin-embedded specimens of NPC were studied by routine LSAB immunohistochemistry. The correlation between their clinical stage and recurrence and these two gene protein expressions was analysed in detail. Results: The positive expression rate of P53 was 68. 57%, and it had no relation with neck lymph node (NLN) metastasis. But the size of metastatic NLN was <4 cm mostly in P53 positive expression of NPC (P<0. 05). The positive expression rate of P53 protein in stage III and IV was higher than that in stage Ⅰ and Ⅱ (P<0. 05), but it was not related with the local recurrence and distant metastasis after radiotherapy. The positive expression rate of c-erbB-2 group was 84. 29%, and it had no correlation with the clinical stage and recurrence. Conclusion: The positive expression rate of P53 protein is high in the advanced stage of NPC, and it is related with the size of metastatic NLN.