山楂化学成分研究

Studies on chemical constituents from fruit of Crataegus pinnatifida

目的 从山里红 Crataegus pinnatifida var.major的成熟干燥果实寻找中药山楂中的专属性成分和降血脂的活性成分。方法 利用多种柱色谱技术进行分离和纯化 ,根据 U V、IR、EI- MS、FAB- MS、1 HNMR、1 3CNMR、HMBC、HMQC和 1 3CGATE等波谱数据分析鉴定结构。结果 从山里红成熟果实中分离鉴定了 6个化合物 ,分别为 :5 ,7,4′-三羟基黄酮 - 8- C-α- L -吡喃鼠李糖基 - (1→ 2 ) -β- D-吡喃葡萄糖苷 ( ) ,即牡荆素鼠李糖苷 (vitexin rham-noside) ,金丝桃苷 (hyperoside, ) ,枸橼酸 (citric acid, ) ,牡荆素 (vitexin, ) ,槲皮素 (quercetin, ) ,熊果酸 (ur-solic acid, )。结论 化合物 为中药山楂中的专属性成分 ,首次由山里红果实中分得 ,化合物

Object To look for the proprietary constituent and the constituents with blood lipid regulating effect from the dried fruit of Crataegus pinnatifida Bge var major N E Br Methods Various column chromatographic techniques were employed for isolation and purification of the constituents UV, IR, EI MS, FAB MS, 1HNMR, 13 CNMR, HMBC, HMQC and 13 CGATE were used to identify the structure of the isolated constituents Results Six compounds were isolated from the fruit of C pinnatifida They were identified as 5, 7, 4′ trihydroxy flavone 8 C α L rhamnopyranosyl (1→2) β D glucopyranoside, i e vitexin rhamnoside (Ⅰ), hyperoside (Ⅱ), citric acid (Ⅲ), vitexin (Ⅳ), quercetin (Ⅴ), ursolic acid (Ⅵ) Conclusion Compound Ⅰ is a proprietary constituent in the fruit of C pinnalifida, which was obtained from this plant for the first time Compound Ⅱ is a main constituent with blood lipid reducing effect in flavonoids