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急性移植物抗宿主病中Ly49A^+T淋巴细胞的分布及其调节 被引量:1

The distribution of Ly49A^+Tlymphocytes and its regulation by cytokine and immunosuppressant in aGVHD
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摘要 目的 :探讨急性GVHD模型中小鼠杀伤性细胞抑制性受体Ly4 9A在造血系统T淋巴细胞亚群的表达及CsA、IL 4、IL 15对Ly4 9A表达的调节 ;方法 :BALB c、C5 7BL 6、CB6F1间脾细胞和骨髓细胞混合移植 ,建立急性GVHD模型 ,利用FCM双色免疫荧光检测 ,动态观察植入后不同时间造血器官T淋巴细胞亚群中Ly4 9A表达情况 ;体外T淋巴细胞培养观察IL 4、IL 15对Ly4 9A表达的调节 ;结果 :①Ly4 9AB6 在B6、F1小鼠的外周血、脾脏和骨髓细胞中均有表达 ,但在胸腺中基本无表达 ;②aGVHD中CD3+ Ly4 9A+ 及CD8+ Ly4 9A+ T细胞在移植早期增加 ,1w后迅速减低 ,至移植后 5w开始升高 ,并可超过移植前 ;CsA明显下调Ly4 9A变化幅度 ;③IL 4、IL 15均有上调脾CD3+ Ly4 9A+ 细胞群的作用 (P =0 0 0 1)。结论 :Ly4 9A+ T细胞主要出现在外周淋巴器官 ;供者Ly4 9A+ T细胞在改变的MHC背景环境下 ,趋向于对宿主的免疫耐受 ,抑制性受体表达增加 ;骨髓的造血微环境可影响骨髓Ly4 9A+ T细胞比例的变化 ; Objective:To investigate the expression profile of Ly49A in a mouse GVHD model and the ex vivo expression alteration of it in T cells exposal to CsA, IL 4, and IL 15, respectively.Methods:Based on mouse aGVHD model, two color fluorescence flow cytometry was used to assay the expression of Ly49A on splenic and bone marrow CD3 +, CD4 + and CD8 + T cell subsets, before and after engraftment in different time interval. Cell culture was used to analyse in vitro Ly49A expression on splenic T cells treated with IL 4 and IL 15.Results:①In normal B6 and F1 strain, the frequencies of Ly49A + cells in BMCs , splenocytes and PMNCs remained at a low level and almost no, if any, such cells were detected in thymus; ②The proportions of CD3 +/Ly49A + andCD8 +/Ly49A + cells rose in early stage(7th,day) , tended to decline until 5 w post engraftment, and thereafter arose and exceed that pretreatment, which was down regulated in the presence of CsA; ③With or without ConA, the CD3 +/Ly49A + proportion were up modulated in purified CD3 +splenic cells, by IL 4 and IL 15 with no different effect demonstrated between those cytokines.Conclusion:KIR +T lymphocytes were most belong to peripheral lymphocytes. There exist a immunological tolerant tendency between grafts and host in semimatched transplant, and some cytokines and immunosuppressants can enhance the tolerance.
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2002年第6期402-406,共5页 Chinese Journal of Immunology
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