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端粒酶基因hTR在大肠癌中的表达及其临床意义 被引量:3

Study on Expression of Telomerase Gene hTR and the Clinical Association in Large Intestinal Cancer
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摘要 目的 探讨大肠癌中端粒酶基因hTR表达与临床参数的关系 ,评价hTR表达检测在大肠癌诊断中的价值。方法 用原位杂交的方法检测端粒酶基因hTR在大肠癌、癌旁组织和大肠良性病变中的表达和分布情况。结果 结果 5 1例大肠癌中hTR基因表达阳性率为 94 .1% (48 5 1) ,5 1例癌旁组织中hTR基因表达阳性率为 0 (0 5 1) ,31例大肠良性病变中hTR基因表达阳性率为 3.2 % (1 31)。大肠癌组织中hTR基因的表达与癌旁组织、大肠良性病变比较差异有显著性 (P均 <0 .0 1)。大肠癌组织中hTR的表达与患者性别、病变部位、病理分类大体类型、淋巴结转移和临床分期无相关性 (P均 >0 .0 5 ) ,但与肿瘤病理分级相关 (P <0 .0 5 )。结论 端粒酶基因hTR表达检测在大肠癌诊断中可能有一定的价值。 Objective To investigate the correlation between the expressions of telomerase gene human telomerase reverse transcriptase(hTR) and clinical parameters in large intestinal cancer,and to evaluate the role of detection of hTR gene in large intestinal cancer diagnosis.Method With in situ hybridization,the expression and distribution of telomerase gene hTR was observed in 51 cases of large intestinal cancer tissues,51 cases of tumoradjacent tissues,31 cases of benign lesions.The results were statisfically analyzed.Result hTR was detected in 94.1%(48/51) of large intestinal cancer.While in the adjacent tissues,the positive rate was 0 (0/51).The positive rate for hTR was 3.2% (1/31) in benign lesions.The positive detection of hTR expression in cancer group significantly different from those in the adjacent tissues and benigh lesions groups(P both<0.01).The expression of hTR correlated well with histological degree (P <0.05),but not with sex,general pathological types,organ,lymph node metastasis and tumor stage (P all>0.05).Conclusion The detection of telomerase hTR gene expression may be useful in diagnosis in large intestinal cancer.
出处 《潍坊医学院学报》 2002年第1期20-22,共3页 Acta Academiae Medicinae Weifang
基金 山东省科学技术攻关计划资助项目 (课题号 :981 1 5450 3B)
关键词 端粒 末端转移酶 HTR基因 大肠癌 基因表达 Telomerase Large intestinal cancer Gene expression hTR gene
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