红花籽亚油酸与油酸成分的同时HPLC-UV快速检测法

HPLC-UV rapid detection of linoleic acid and oleic acid in safflower seeds

建立一种利用高效液相色谱法‐紫外法快速、简单并同时检测红花籽中油酸与亚油酸成分含量的方法,用以直接鉴定红花籽亚油酸和油酸含量高低,为红花资源鉴定提供理论依据。采用Outstand C18 HPLC Column色谱柱,流动相为乙腈‐0. 1%磷酸水溶液(85∶15),检测波长为203 nm,流速为1 mL/min,柱温为30℃。红花种子中亚油酸的理论塔板数为24 620,油酸的理论塔板数为25 842。结果亚油酸和油酸分别在0. 110~2. 205、0. 872~17. 444 g/L,与峰面积呈良好的线性关系,亚油酸加样回收率在99%,油酸加样回收率达到100%,亚油酸RSD与油酸RSD均为0%(n=3);亚油酸与油酸的质量分数分别是12. 32%,14. 07%,13. 92%和0. 82%,0. 93%,0. 95%,RSD分别为1. 22%,0. 94%(n=3)。该方法具有分离效率高,操作简单,检测快速的优点。

To establish a rapid,simple and simultaneous method for the content determination of oleic acid and linoleic acid in safflower seeds by high-performance liquid chromatography-ultraviolet method,and to provide a theoretical basis for the identification of safflower resources,a chromatographic process was set up by using Outstand C18 HPLC Column,and a mobile phase of 85%acetonitrile-15%phosphoric acid solution(0.1%)was used with a flow rate at1.0 mL/min,column temperature at 30℃,and determination wavelength at 203 nm.The results showed that the theoretical plate number of linoleic acid in safflower seeds is 24 620,and the theoretical plate number of oleic acid is 25 842.The calibration curve of oleic acid was linear between 0.8722~17.444 g·L-1,and linoleic acid was linear between 0.110~2.205 g/L,with the average recovery 99%-100%and RSD of 0%(n=3).The contents of oleic acid and linoleic acid in safflower seeds were 12.32%,14.07%,13.92%and 0.82%,0.93%,0.95%,and the RSD was 1.22%,0.94%(n=3),respectively.The advantages of the method are high separation efficiency,simple operation,and rapid detection.