鱼粉粗蛋白含量近红外模型的建立

Establishment of NIRS Model for Determining the Content of Crude Protein in Fish Meal

[目的]为鱼粉粗蛋白含量提供一种快速、低廉、准确可靠的分析方法。[方法]以国内常见鱼粉为材料,使用InfraXact Lab型近红外光谱分析仪测定鱼粉的近红外光谱值,并采用常规凯氏定氮法测定鱼粉样品中粗蛋白含量,建立鱼粉粗蛋白含量的近红外分析模型,并对模型测定结果进行预测准确性评价。[结果]鱼粉粗蛋白含量定标方程的SECV值为0.515,1-VR值为0.865,粗蛋白含量的验证参数SEC值为0.302,RSQ值为0.925,说明定标方程的预测能力较好,可用来进行鱼粉粗蛋白含量的测定。[结论]建立的鱼粉粗蛋白含量近红外分析模型具有一定的实用价值,可用于鱼粉常规养分分析的实际工作。

[Objective] The research aimed to provide a rapid,cheap and reliable determination method for the content of crude protein in fish meal.[Method] Taking common domestic fish meal as test materials,the near infrared spectrum value of fish meal was determined by using InfraXact Lab near-infrared spectroscopy analyzer and the content of crude protein in fish meal samples was determined by using conventional Kjeldahl method.NIRS model for the determination of crude protein content in fish meal was established and the prediction accuracy of the determination results by using NIRS model was evaluated.[Result] The standard error of cross-validation （SECV）,cross-validation correlation coefficient （1-VR）,standard error of calibration （SEC）,and egression squared （RSQ） of the calibration equation for the determination of crude protein content in fish meal were 0.515,0.865,0.302 and 0.925,respectively.It was proved that the calibration equation had better prediction ability,so it could be used for the determination of crude protein content in fish meal.[Conclusion] The established NIRS model for the determination of crude protein content in fish meal had certain application value,so it could be used in the conventional nutrient analysis of fish meal in practice.