P2X4受体在大鼠脊髓小胶质细胞中的表达

Expression of P2X4 Receptor in Microglia from Spinal of Rats with Neuropathic Pain

目的 研究神经病理性疼痛大鼠脊髓小胶质细胞P2X4受体的表达及意义.方法 40只健康SD大鼠随机分为脊神经结扎组（SNL组n=20）及对照组（Con组n=20）.SNL组结扎腰5脊神经,对照组仅暴露脊神经后不结扎立即缝合.术后观测2组机械痛阀值的变化,SNL组于术后第1、7、14、21 d处死4只大鼠,取L4～6段脊髓,用免疫组化及Western印迹法测定分析P2X4受体及OX-42的表达.结果 SNL组P2X4表达对照组显著增加.结论 神经病理性疼痛大鼠脊髓小胶质细胞P2X4受体表达明显增加.

Objective To investigate the expression of P2X4 receptor in microglia from spinal of rats with neuropathic pain.Methods Forty healthy male SD rats weighted 180 ～ 240 g were randomly divided into 2 groups （n =20） ; SNL group,the spinal were ligated,the control group,the spinal nerve were exposed but not ligated.The PWT were mesured in all rats.The rats of SNL group were killed separately on 1day,7 day,14 day and 21 day after surgery.The L4-6 spinal samples were taken and the expression of P2X4 was detected by immunohistochemistry and Westrn blot.Result Compared with the control group,the expression levels of P2X4 receptor were increased singnificantly in SNL group.Conclusion In the rats with neuropathic pain,the expression of P2X4 receptor in spinal microglia is upregulated.