HPLC法同时测定乌杞明目口服液中6种成分

Simultaneous determination of six constituents in Wuqi Mingmu Oral Liquid by HPLC

目的建立HPLC双波长法同时测定乌杞明目口服液(赤芍、蒲黄、制何首乌、三七、丹参等)中芍药苷、香蒲新苷、2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷、人参皂苷Rg1、丹酚酸B和人参皂苷Rb1的方法。方法采用Kromasil C18柱,以乙腈-0.1%磷酸水为流动相,梯度洗脱,体积流量1.0 mL/min,双波长检测(λ1=203 nm、λ2=230 nm),柱温25℃。结果芍药苷在4.799～57.59μg/mL(r=0.999 3)、香蒲新苷在1.250～15.00μg/mL(r=0.999 5)、2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷在1.477～17.73μg/mL(r=0.999 1)、人参皂苷Rg1在7.781～46.68μg/mL(r=0.999 4)、丹酚酸B在4.775～57.30μg/mL(r=0.999 1)、人参皂苷Rb1在3.734～44.81μg/mL(r=0.999 7)范围内线性关系良好,平均回收率分别为98.1%、98.9%、99.6%、101.3%、101.9%、97.7%,RSD分别为1.7%、1.1%、1.0%、0.6%、1.4%、1.4%。结论本方法色谱峰分离效果和峰形均良好,可作为该口服液的质量控制方法。

AIM To develop an HPLC method for simultaneously determining the contents of paeoniflorin, typhaneoside, 2,3,5,4＇-tetrahydroxystilbene-2-O-15-D-glucoside, ginsenoside Rgl, salvianolic acid B, and gin- senoside Rb1 in Wuqi Mingmu Oral Liquid （Paeoniae Radix rubra, Typhae Pollen , Polygoni multiflori Radix praeparata, Notoginseng Radix et Rhizoma, Salviae miltiorrhizae Radix et Rhizoma, etc ）. METHODS A Kromasil C,s column （250 mm ×4.6 ram, 5 μm） was used with the mobile phase consisted of 0. 1% phosphoric acid and acetonitrile in linear gradient elution mode. The flow rate was 1.0 mL/min. The wavelength was set at 203 nm and 230 nm with 25 ℃ column temperature. RESULTS The experiment results showed the good line- arities in the range of 4. 799 - 57.59 I^g/mL （ r = 0. 999 3 ） for paeoniflorin, 1. 250 - 15.00 μg/mL （ r = 0. 999 5 ） for typhaneoside, 1. 477 - 17.73 μg/mL （r = 0. 999 1 ） for 2,3,5,4＇-tetrahydroxystilhene-2-O-13-D- glucoside, 7. 781 -46.68 Ixg/mL （r =0. 999 4） for ginsenoside Rgl, 4. 775 -57.30 μg/mL ：（r =0. 999 1 ） for salvianolic acid B, 3. 734 -44. 81 μg/mL （r =0. 999 7） for ginsenoside Rb,, respectively. The average re- covery rates of the six investigated compounds were 98.1% , 98.9% , 99.6% , 101.3% , 101.9% , and 97.7% , and RSDs were 1.7% , 1.1% 1.0% , 0.6% , 1.4% , and 1.4% , respectively. CONCLU-SION The developed method is good for peak separation and shapes, and can be applied for quality control of the preparation.