血小板衍生生长因子-BB、转化生长因子-β_1联合应用对大鼠正畸牙牙周膜中整合素β_3表达的影响

Synergistic effect of platelet-derived growth factor-BB and transforming growth factor-β_1 on expression of integrin β_3 in periodontal membrane of rat orthodontic tooth

目的观察大鼠正畸牙局部联合应用血小板衍生生长因子-BB(PDGF-BB)和转化生长因子-β1(TGF-β1)后正畸牙牙周膜中整合素β3表达的变化。方法按随机数字表法,将32只大鼠随机均分为4组。建立大鼠上颌第一磨牙近中移动正畸模型,隔日于施力磨牙颊侧牙龈黏膜下分别注射1%PBS、10 ng PDGF-BB、5 ng TGF-β1、10 ng PDGF-BB和5 ng TGF-β1叠加剂量,注射体积均为0.1 mL。加力10 d后处死动物,取左上颌第一磨牙及其牙周组织,用免疫组织化学方法检测牙周组织中整合素β3的表达,进行阳性表达区域平均光密度值测量,对测量结果行方差分析,服从正态分布,再行组间t检验统计学分析。结果各实验组压力侧牙周膜细胞中整合素β3的表达强度均高于对照组(P<0.01),其中PDGF-BB+TGF-β1组与TGF-β1组或PDGF-BB组比较差异均有统计学意义(P<0.01)。实验组张力侧牙周膜细胞中整合素β3的表达强度均高于对照组(P<0.05),其中PDGF-BB+TGF-β1组整合素β3表达强度较强,与TGF-β1或PDGF-BB组比较差异无统计学意义(P>0.05)。结论局部联合应用外源性PDGF-BB和TGF-β1对大鼠正畸牙牙周组织中整合素β3的上调作用较单独运用PDGF-BB或TGF-β1组强,二者联合应用有协同效应,共同促进了正畸牙周组织改建。

Objective To investigate the synergistic effect of transforming growth factor-β1 （TGF-β1） and platelet-derived growth factor-BB （PDGF-BB） on the expression of integrin β3 in periodontal membrane of rat orthodontic tooth. Methods An orthodontic tooth movement model was established. Up to 32 experimental rats were randomly divided into four groups according to a random number table. The four groups were injected with 1%PBS, TGF-β1 （5 ng）, PDGF-BB （10 ng）, and combined TGF-β1 （5 ng） and PDGF-BB （10 ng） in the buccal submucosal, respectively. The volume injected in each group was 0.1 mL. The animals were then sacrificed on the 10th day. The leit maxillary first molar and periodontal tissue were taken. Different expressions of integrin β3 were detected in periodontal tissues through immunohistochemistry. Mean optical density （OD） values of the positive fields were examined. The data obtained were analyzed through ANOVA. The data followed normal distribution, and were compared via t-test. Results Compared with the control groups, the expression of integrin ~3 was higher in the experimental gronpin tension sides （P〈0.01）. Significant differences in tension sides between the single- injection groups and the combined group were observed （P〈0.01）. Compared with the control groups, the expression ofinte-grin 133 was higher in the experimental group in compression sides （P〈0.05）. In addition, there was no significant diffe- rences in compression sides between the single-injection groups and the combined group （P〉0.05）. Conclusion In terms of local regulatory factors, TGF-β1 combined withPDGF-BB enhance the expression of integrin 63 in the periodontal membrane and synergistic effect of the two growth factors is better than the single growth factor.