期刊文献+

甲型副伤寒沙门菌培养条件的优化 被引量:2

Optimization on growth condition of Salmonella paratyphoid A
下载PDF
导出
摘要 优化甲型副伤寒沙门菌的培养条件,提高菌体产量。方法通过单因素及正交试验,对影响甲型副伤寒沙门菌生长的培养温度、NaCl浓度和pH等条件进行优化。结果甲型副伤寒沙门菌在NaCl浓度0.75%、温度35℃、pH6.5时菌体产量最高。结论通过对甲型副伤寒沙门菌培养条件进行优化,获得较高的菌体产量,为后期诊断试剂盒的开发奠定了基础。 Objective To optimize the growth condition of Salmonella paratyphoid A. Methods To study the effect for temperature ,pH and NaCl concentration on the growth of Salmonella paratyphoid A by using the method of orthogonal design. Results The optimal culture condition of Salmonella paratyphoid A is NaCl concentration 0.75% , temperature 35 ℃ and pH 6.5. Conclusion The optimal culture condition of Salmonella paratyphoid A is successfully achieved in this investigation.
出处 《微生物学免疫学进展》 2014年第4期26-28,共3页 Progress In Microbiology and Immunology
关键词 甲型副伤寒沙门菌 优化培养 Salmonella paratyphoid A Optimization for cuhivation
  • 相关文献

参考文献2

二级参考文献25

  • 1Clump JA, Luby SP, Mintz ED. The global burden of typhoid fever[ J]. Bull World Health Organ ,2004,82 ( 5 ) :346 - 353.
  • 2Ochiai RL, Wang X, von Seidlein L, et al, Salmonella paratyphi A rates, Asia [ J ]. Emerg Infect Dis, 2005,11 ( 11 ) : 1764 - 1766.
  • 3Yang HH, Gong J, Zhang J, et al. An outbreak of Salmonella paratyphi A in a boarding school: a community-acquired enteric fever and carriage investigation [ J ]. Epidemiol Infect, 2010,138(12) :1765 - 1774.
  • 4Woods CW, Murdoch DR, Zimmerman MD, et al. Emergence of Salmonella enterica serotype paratyphi A as a major cause of enteric fever in Kathmandu, Nepal [ J ]. Trans R Soc Trop Med Hyg ,2006 ,100 ( 11 ) : 1063 - 1067.
  • 5Connor BA, Schwartz E. Typhoid and paratyphoid fever in travelers[J]. Lancet Infect Dis,2005,5 ( 10 ) :623 - 628.
  • 6Meltzer E, Sadik C, Schwartz E. Enteric fever in Israeli travelers : a nationwide study [J]. J Travel Med, 2005,12 ( 5 ) : 275 - 281.
  • 7Verma S, Thakur S, Kanga A, et al. Emerging Salmonella paratyphi A enteric fever and changing trends in antimicrobial resistance pattern of Salmonella in Shimla [ J ]. Indian J Med Microbiol,2010,28( 1 ) :51 -53.
  • 8Molloy A, Nair S, Cooke FJ, et al. First report of Salmonella enterica serotype paratyphi a azithromycin resistance leading to treatment failure. 2010[J]. J Clin Microbiol,2010, 48 (12) : 4655 - 4657.
  • 9Guzman CA, Borsutzky S, Griot-Wenk M, et al. Vaccines against typhoid fever [J]. Vaccine, 2006,24 ( 18 ) : 3804 - 3811.
  • 10Levine MM, Ferreccio C, Black RE, et al. Ty21a live oral typhoid vaccine and prevention of paratyphoid fever caused by Salmonella enterica serovar paratyphi B [J]. Clin Infect Dis, 2007,45 Suppl 1 :S24 -28.

同被引文献22

  • 1霍向东,石玉瑚.大肠杆菌高密度发酵补料调控策略的研究进展[J].新疆农业科学,2004,41(F08):16-18. 被引量:7
  • 2孙玉岭,吴斌,秦成,陈明生.食品中沙门菌培养条件的研究[J].中国微生态学杂志,2006,18(1):48-48. 被引量:7
  • 3许一平,成炜,邵彦春,陈福生.沙门菌、大肠杆菌和金黄色葡萄球菌的多重PCR检测[J].微生物学通报,2006,33(6):89-94. 被引量:50
  • 4邵碧英,陈彬,汤敏英,吴谦,张体银.沙门氏菌DNA提取及PCR反应条件的优化[J].食品科学,2007,28(7):331-334. 被引量:29
  • 5Cheng Y, Liu Y, Huang J, et al. Combining biofunctional magnetic nanoparticles and ATP bioluminescence for rapid detection of Escherichia coli[J]. Talanta, 2009, 77(4): 1332-1336.
  • 6Valat C, Champiat D, N'Guyen TT, et al. Use of ATP bioluminescence to determine the bacterial sensitivity threshold to a bacteriocin[J]. Luminescence, 2003, 18(5): 254-258.
  • 7Wu H, Zuo Y, Cui C, et al. Rapid quantitative detection of Brucella melitensis by a label-free impedance immunosensor based on a gold nanoparticle-modified screen-printed carbon electrode [J]. Sensors (Basel), 2013, 13(7): 8551-8563.
  • 8Torres-Chavolla E, Alocilja EC. Aptasensors for detection of microbial and viral pathogens [J]. Biosens Bioelectron, 2009, 24(11): 3175-3182.
  • 9Van Nevel S, Koetzsch S, Weilenmann HU, et al. Routine bacterial analysis with automated flow cytometry [J]. J Microbiol Methods, 2013, 94(2): 73-76.
  • 10Mehta PK, Raj A, Singh NP, et al. Detection of potential microbial antigens by immuno-PCR (PCR-amplified immunoassay)[J]. J Med Microbiol, 2014, 63(5): 627-641.

引证文献2

二级引证文献4

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部