桂皮醛对人膝骨关节炎滑膜成纤维细胞增殖的影响

The Effect of Cinnamaldehyde on Proliferation of Synovioblast in Patients with Osteoarthritis

目的：探讨不同浓度桂皮醛对人膝关节骨性关节炎（KOA）滑膜成纤维细胞的影响。方法：关节镜术中取KOA患者验证滑膜组织用I型胶原酶消化后进行原代培养，取传代3～5代的滑膜成纤维细胞进行不同浓度桂皮醛药物干预。应用四唑盐（MTT）法检测KOA滑膜细胞的增殖水平。结果：中高浓度桂皮醛作用24、48和72h，能明显抑制人KOA滑膜成纤维细胞的增殖（P〈O．05），且呈时间、剂量依赖性。结论：中高浓度桂皮醛能明显抑制KOA炎性滑膜细胞的增殖活性而较低浓度桂皮醛能促进KOA炎性滑膜细胞的增殖。

Objective：To explore the effect of cinnamaldehyde on proliferation of synovioblast in patients with knee osteoar- thritis. Methods： Synovioblast in patients with osteoarthritis were cultured and identified. After 3～5 generation incuba- tions, the synovial membrane were incubated with different dosage and evaluated by MTT. Results：The optical density were significantly different between experimental group and control group（P〈0.05）for 24,48 and 72 hours with a dependence of time and dose. Conclusion： Low dose of cinnamaldehyde promote proliferation of synovioblast in patients with osteoarthritis whereas middle and high supress the proliferation.