远志药材及其混伪品的DNA条形码鉴定

Identification of Polygalae Radix and Its Adulterants by psbA-trnH Sequence

目的:对中药材远志及其混伪品进行分子鉴定,保障药材质量及用药安全。方法:对46份样品进行DNA提取,通过聚合酶链式反应(PCR)扩增其psbA-trnH序列并双向测序,应用CodonCode Aligner V3.7.1对测序峰图校对拼接,去除低质量区和引物区,得到psbA-trnH序列,用MEGA 6.0对序列进行比对分析,基于K2P遗传距离构建系统聚类树。结果:通过相似性搜索法能准确鉴别远志及其混伪品,远志两个基原的种内最大K2P距离分别为0.004和0,均小于其与混伪品的种间最小K2P遗传距离(0.010和0.005),基于psbA-trnH序列的系统发育树可将远志药材及其混伪品明显区分开。结论:psbA-trnH序列能有效鉴别远志药材及其混伪品,为中药材的质量控制提供新方法。

In this study,Polygalae radix and its adulterants were identified by psbA-trnH sequence.The genomic DNA was extracted from forty-six samples, the psbA-trnH sequences were amplified and sequenced Bi-directionally, and then assembled sequences by Codoncode Aligner V 3.7.1. The genetic distances were computed by kimura 2-parameter （K2P） model, and the Neighbor-Joining tree was constructed by MEGA 6.0. Results showed that minimum intra-specific K2P distance of Polygala tenuifolia and Polygala sibirica were 0.004 and 0, which were smaller than the maximum intra-specific K2P. The NJ tree showed Polygalae radix can be distinguished from its adulterants by psbA-trnH sequences. Therefore, using psbA-trnH sequences can distinguish Polygalae radix from its adulterants.